1q32

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[[Image:1q32.gif|left|200px]]
 
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==Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase==
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The line below this paragraph, containing "STRUCTURE_1q32", creates the "Structure Box" on the page.
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<StructureSection load='1q32' size='340' side='right'caption='[[1q32]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1q32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q32 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q32 OCA], [https://pdbe.org/1q32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q32 RCSB], [https://www.ebi.ac.uk/pdbsum/1q32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q32 ProSAT]</span></td></tr>
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{{STRUCTURE_1q32| PDB=1q32 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TYDP1_YEAST TYDP1_YEAST] DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Cleaves also 5' phosphotyrosyl adducts resulting from dead-end complexes between DNA and the active site tyrosine of topoisomerase II. Contributes to DNA repair after radiation damage. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).<ref>PMID:10521354</ref> <ref>PMID:16751265</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q3/1q32_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q32 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase'''
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==See Also==
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*[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the resolution of 3' and 5' phospho-DNA adducts. A defective mutant, associated with the recessive neurodegenerative disease SCAN1, accumulates Tdp1-DNA complexes in vitro. To assess the conservation of enzyme architecture, a 2.0 A crystal structure of yeast Tdp1 was determined that is very similar to human Tdp1. Poorly conserved regions of primary structure are peripheral to an essentially identical catalytic core. Enzyme mechanism was also conserved, because the yeast SCAN1 mutant (H(432)R) enhanced cell sensitivity to the DNA topoisomerase I (Top1) poison camptothecin. A more severe Top1-dependent lethality of Tdp1H(432)N was drug-independent, coinciding with increased covalent Top1-DNA and Tdp1-DNA complex formation in vivo. However, both H(432) mutants were recessive to wild-type Tdp1. Thus, yeast H(432) acts in the general acid/base catalytic mechanism of Tdp1 to resolve 3' phosphotyrosyl and 3' phosphoamide linkages. However, the distinct pattern of mutant Tdp1 activity evident in yeast cells, suggests a more severe defect in Tdp1H(432)N-catalyzed resolution of 3' phospho-adducts.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1Q32 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q32 OCA].
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==Reference==
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Mutation of a conserved active site residue converts tyrosyl-DNA phosphodiesterase I into a DNA topoisomerase I-dependent poison., He X, van Waardenburg RC, Babaoglu K, Price AC, Nitiss KC, Nitiss JL, Bjornsti MA, White SW, J Mol Biol. 2007 Sep 28;372(4):1070-81. Epub 2007 Aug 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17707402 17707402]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Babaoglu K]]
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[[Category: Babaoglu, K.]]
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[[Category: He X]]
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[[Category: He, X.]]
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[[Category: Nitiss JL]]
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[[Category: Nitiss, J L.]]
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[[Category: Nitiss KC]]
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[[Category: Nitiss, K C.]]
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[[Category: Price A]]
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[[Category: Price, A.]]
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[[Category: White SW]]
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[[Category: White, S W.]]
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[[Category: Dna repair]]
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[[Category: Hydrolase]]
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[[Category: Replication]]
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[[Category: Tdp]]
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[[Category: Transcription]]
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[[Category: Tyrosyl-dna phosphodiesterase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:53:29 2008''
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Current revision

Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase

PDB ID 1q32

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