2gfb

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{{STRUCTURE_2gfb| PDB=2gfb | SCENE= }}
{{STRUCTURE_2gfb| PDB=2gfb | SCENE= }}
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'''CRYSTAL STRUCTURE OF A CATALYTIC FAB HAVING ESTERASE-LIKE ACTIVITY'''
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===CRYSTAL STRUCTURE OF A CATALYTIC FAB HAVING ESTERASE-LIKE ACTIVITY===
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==Overview==
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BACKGROUND: Antibodies with catalytic properties can be prepared by eliciting an antibody response against 'transition state analog' haptens. The specificity, rate and number of reaction cycles observed with these antibodies more closely resemble the properties of enzymes than any of the many other known enzyme-mimicking systems. RESULTS: We have determined to 3 A resolution the first X-ray structure of a catalytic antibody Fab. This antibody catalyzes the hydrolysis of a p-nitrophenyl ester. In conjunction with binding studies in solution, this structure of the uncomplexed site suggests a model for transition state fixation where two tyrosines mimic the oxyanion binding hole of serine proteases. A comparison with the structures of known Fabs specific for low molecular weight haptens reveals that this catalytic antibody has an unusually long groove at its combining site. CONCLUSION: Since transition state analogs contain elements of the desired product, product inhibition is a severe problem in antibody catalysis. The observation of a long groove at the combining site may relate to the ability of this catalytic antibody to achieve multiple cycles of reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 8069632 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8069632}}
==About this Structure==
==About this Structure==
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[[Category: Knossow, M.]]
[[Category: Knossow, M.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:02:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:05:35 2008''

Revision as of 17:05, 28 July 2008

Image:2gfb.png

Template:STRUCTURE 2gfb

CRYSTAL STRUCTURE OF A CATALYTIC FAB HAVING ESTERASE-LIKE ACTIVITY

Template:ABSTRACT PUBMED 8069632

About this Structure

2GFB is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of a catalytic antibody Fab with esterase-like activity., Golinelli-Pimpaneau B, Gigant B, Bizebard T, Navaza J, Saludjian P, Zemel R, Tawfik DS, Eshhar Z, Green BS, Knossow M, Structure. 1994 Mar 15;2(3):175-83. PMID:8069632

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