User:Udayan Shevade/Sandbox1
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | = SV40 Large T | + | = SV40 Large T Antigen = |
=== Introduction === | === Introduction === | ||
| - | The SV40 large T | + | The SV40 large T antigen is a multifunctional regulatory protein encoded by Simian Virus 40, belonging to the AAA+ family of helicases <ref name="A">PMID:8946857</ref>. It is responsible for initiation of DNA replication, regulation of transcription and alteration of the host cell cycle to promote infectivity. Large T antigen is an early gene product of SV40 and is translated via differential mRNA splicing. |
| Line 10: | Line 10: | ||
T-antigen consists of an N-terminal J domain, a central origin-binding domain, and a C-terminal helicase domain <ref name="A"/>. | T-antigen consists of an N-terminal J domain, a central origin-binding domain, and a C-terminal helicase domain <ref name="A"/>. | ||
| - | <StructureSection load=' | + | <StructureSection load='2FUF' size='500' side='right' caption='The origin binding domain of SV40 T antigen (PDB entry [[2FUF]])'> |
==== The Origin Binding Domain ==== | ==== The Origin Binding Domain ==== | ||
| - | The central obd monomer consists of | + | The central obd monomer consists of a five-strand anti-parallel beta sheet flanked by two pairs of alpha helices. These molecules arrange tightly into a hexameric left-handed spiral, with 6 obd's per turn. The conformation creates a central channel 60 Angstrom wide, large enough for dsDNA, and is highly positively charged <ref name="A"/>. Side-side interaction is crucial in hexamerization, for which residues <scene name='User:Udayan_Shevade/Sandbox1/Obd/2'>Phe 183 and Ser 185</scene> are crucial. Along the inner surface of the channel, residues implicated in DNA binding are <scene name='User:Udayan_Shevade/Sandbox1/Obd_153_154_155/3'>Asn 153, Arg 154, Thr 155 from the A motif</scene>; His 203, Arg 204 from the B2 motif; as well as His 201 and Arg 202. </StructureSection> |
Revision as of 17:21, 10 November 2011
SV40 Large T Antigen
Introduction
The SV40 large T antigen is a multifunctional regulatory protein encoded by Simian Virus 40, belonging to the AAA+ family of helicases [1]. It is responsible for initiation of DNA replication, regulation of transcription and alteration of the host cell cycle to promote infectivity. Large T antigen is an early gene product of SV40 and is translated via differential mRNA splicing.
Structure
T-antigen consists of an N-terminal J domain, a central origin-binding domain, and a C-terminal helicase domain [1].
| |||||||||||
