Journal:Acta Cryst D:S2059798318014900
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A ‘branched-ligand’ AliC complex was obtained through co-crystallization, with crystals forming in a new space group. This form diffracted poorly and data could only be obtained to 2.95 Å resolution [[6gya]]). Weak density in the -1 subsite, largely diffuse but greater than would be expected for discrete solvent, remained unmodelled. Density was clearer for a panose trisaccharide with an α-1,4-linked disaccharide in subsites +1 and +2 and, crucially, clear density for an α-1,6 branch accommodated in the +1 subsite, providing a structural context for the limit digest analysis of action on amylopectin starch. The <scene name='79/799580/Cv/15'>binding of the branched oligosaccharide in subsites +1, +2 and +1'</scene> (<span style="color:lime;background-color:black;font-weight:bold;">oligosaccharide colored in green</span>). | A ‘branched-ligand’ AliC complex was obtained through co-crystallization, with crystals forming in a new space group. This form diffracted poorly and data could only be obtained to 2.95 Å resolution [[6gya]]). Weak density in the -1 subsite, largely diffuse but greater than would be expected for discrete solvent, remained unmodelled. Density was clearer for a panose trisaccharide with an α-1,4-linked disaccharide in subsites +1 and +2 and, crucially, clear density for an α-1,6 branch accommodated in the +1 subsite, providing a structural context for the limit digest analysis of action on amylopectin starch. The <scene name='79/799580/Cv/15'>binding of the branched oligosaccharide in subsites +1, +2 and +1'</scene> (<span style="color:lime;background-color:black;font-weight:bold;">oligosaccharide colored in green</span>). | ||
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| + | '''PDB references:''' Amylase in complex with acarbose [[6gxv]]; Amylase in complex with branched ligand [[6gya]]. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 13:04, 13 June 2022
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