Triose Phosphate Isomerase
From Proteopedia
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{{STRUCTURE_1tim | PDB=1tim | SCENE=1}} | {{STRUCTURE_1tim | PDB=1tim | SCENE=1}} | ||
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| - | Triose Phosphate Isomerase is a dimeric protein of two identical subunits. Each subunit contains 8 alpha helices <scene name='Triose_Phosphate_Isomerase/Helices/1'>8 exterior helices</scene> surrounding 8 interior beta sheets. | + | Triose Phosphate Isomerase is a dimeric protein of two identical subunits. Each subunit contains 8 alpha helices <scene name='Triose_Phosphate_Isomerase/Helices/1'>8 exterior helices</scene> surrounding 8 interior <scene name='Triose_Phosphate_Isomerase/Beta_sheet/1'>beta sheets</scene>, which form the TIM barrels found in each respective subunit. |
== Disease == | == Disease == | ||
Revision as of 15:33, 21 March 2009
Triose Phosphate Isomerase is a dimeric protein of two identical subunits. Each subunit contains surrounding 8 interior
Contents |
Overview
(TPI or TIM) which catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate, an essential process in the glycolytic pathway.
Mechanism
TPI catalyzes the transfer of a hydrogen atom from carbon 1 to carbon 2, an intramolecular oxidation-reduction. This isomerization of a ketose to an aldose proceeds through an enediol intermediate.<Biochemistry 6th Edition>
Acid Base Catalysis
TPI carries out the isomerization reaction through acid base chemistry involving
These two catalytic residues include which plays the role of the general base catalyst in a proton abstraction mechanism as it abstracts a proton from carbon 1, which then donates it to carbon 2. Glutamate 165 requires , the general acid which donates a proton to the C-1 carbonyl oxygen.
Structure & Function
Triose Phosphate Isomerase is a dimeric protein of two identical subunits. Each subunit contains 8 alpha helices surrounding 8 interior , which form the TIM barrels found in each respective subunit.
Disease
Triose Phosphate Isomerase Deficiency
See Also
References
Triose Phosphate Isomerase [[1]] Triose Phosphate Isomerase Deficiency [[2]]
Proteopedia Page Contributors and Editors (what is this?)
Gregg Snider, Eric Martz, Michal Harel, Alexander Berchansky, David Canner, Eran Hodis, Stephen Everse, Angel Herraez, Jane S. Richardson
