1ukv
From Proteopedia
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Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase
Overview
Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the guanosine diphosphate (GDP) dissociation inhibitor (RabGDI). Using a combination of chemical synthesis and protein engineering, we generated and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of the complex was solved to 1.5 angstrom resolution and provides a structural basis for the ability of RabGDI to inhibit the release of nucleotide by Rab proteins. Isoprenoid binding requires a conformational change that opens a cavity in the hydrophobic core of its domain II. Analysis of the structure provides a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans.
About this Structure
1UKV is a Protein complex structure of sequences from Saccharomyces cerevisiae with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase., Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, Waldmann H, Goody RS, Alexandrov K, Science. 2003 Oct 24;302(5645):646-50. PMID:14576435
Page seeded by OCA on Thu Feb 21 15:25:37 2008
