2jbv

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Image:2jbv.png

Template:STRUCTURE 2jbv

CRYSTAL STRUCTURE OF CHOLINE OXIDASE REVEALS INSIGHTS INTO THE CATALYTIC MECHANISM

Template:ABSTRACT PUBMED 18072756

About this Structure

2JBV is a 2 chains structure of sequences from Arthrobacter globiformis. Full crystallographic information is available from OCA.

Reference

  • Quaye O, Lountos GT, Fan F, Orville AM, Gadda G. Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase. Biochemistry. 2008 Jan 8;47(1):243-56. Epub 2007 Dec 12. PMID:18072756 doi:10.1021/bi7017943
  • Fan F, Ghanem M, Gadda G. Cloning, sequence analysis, and purification of choline oxidase from Arthrobacter globiformis: a bacterial enzyme involved in osmotic stress tolerance. Arch Biochem Biophys. 2004 Jan 1;421(1):149-58. PMID:14678796
  • Gadda G. Kinetic mechanism of choline oxidase from Arthrobacter globiformis. Biochim Biophys Acta. 2003 Mar 21;1646(1-2):112-8. PMID:12637017
  • Gadda G, Powell NL, Menon P. The trimethylammonium headgroup of choline is a major determinant for substrate binding and specificity in choline oxidase. Arch Biochem Biophys. 2004 Oct 15;430(2):264-73. PMID:15369826 doi:10.1016/j.abb.2004.07.011
  • Fan F, Gadda G. On the catalytic mechanism of choline oxidase. J Am Chem Soc. 2005 Feb 23;127(7):2067-74. PMID:15713082 doi:10.1021/ja044541q
  • Ghanem M, Gadda G. On the catalytic role of the conserved active site residue His466 of choline oxidase. Biochemistry. 2005 Jan 25;44(3):893-904. PMID:15654745 doi:10.1021/bi048056j
  • Ghanem M, Gadda G. Effects of reversing the protein positive charge in the proximity of the flavin N(1) locus of choline oxidase. Biochemistry. 2006 Mar 14;45(10):3437-47. PMID:16519539 doi:10.1021/bi052514m

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