1zfo
From Proteopedia
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AMINO-TERMINAL LIM-DOMAIN PEPTIDE OF LASP-1, NMR
Overview
The three-dimensional solution structure of the 1:1 complex between the, synthetic peptide ZF-1 and zinc was determined by 1H NMR spectroscopy. The, peptide, initially isolated from pig intestines, is identical in sequence, to the 30 N-terminal amino acid residues of the human protein Lasp-1, belonging to the LIM domain protein family. The final set of 20, energy-refined NMR conformers has an average rmsd relative to the mean, structure of 0.55 A for the backbone atoms of residues 3-30. Calculations, without zinc atom constraints unambiguously identified Cys 5, Cys 8, His, 26, and Cys 29 as the zinc-coordinating residues. LIM domains consist of, two sequential zinc-binding modules and the NMR structure of the ZF-1-zinc, complex is the first example of a structure of an isolated module., Comparison with the known structures of the N-terminal zinc-binding, modules of both the second LIM domain of chicken CRP and rat CRIP with, which ZF-1 shares 50% and 43% sequence identity, respectively, supports, the notion that the zinc-binding modules of the LIM domain have a, conserved structural motif and identifies local regions of structural, diversity. The similarities include conserved zinc-coordinating residues, a rubredoxin knuckle involving Cys 5 and Cys 8, and the coordination of, the zinc ion by histidine N delta in contrast to the more usual, coordination by N epsilon observed for other zinc-finger domains. The, present structure determination of the ZF-1-zinc complex establishes the, N-terminal half of a LIM domain as an independent folding unit. The, structural similarities of N- and C-terminal zinc-binding modules of the, LIM domains, despite limited sequence identity, lead to the proposal of a, single zinc-binding motif in LIM domains. The coordinates are available, from the Brookhaven protein data bank, entry 1ZFO.
About this Structure
1ZFO is a Single protein structure of sequence from Sus scrofa with ZN and ACE as ligands. Full crystallographic information is available from OCA.
Reference
Solution structure of a naturally-occurring zinc-peptide complex demonstrates that the N-terminal zinc-binding module of the Lasp-1 LIM domain is an independent folding unit., Hammarstrom A, Berndt KD, Sillard R, Adermann K, Otting G, Biochemistry. 1996 Oct 1;35(39):12723-32. PMID:8841116
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