413d

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Template:STRUCTURE 413d

A'-FORM RNA DOUBLE HELIX IN THE SINGLE CRYSTAL STRUCTURE OF R(UGAGCUUCGGCUC)

Overview

Here we demonstrate the presence of the A'-RNA conformation using the single crystal structure of a tridecamer: r(UGAGCUUCGGCUC). The average A'-RNA conformation deduced from X-ray fiber diffraction data had only been available previously, but now the presence of the A'-RNA conformation has been found in a single crystal structure for the first time. Statistical analysis showed that the A'-RNA conformation is distinguishable from the A-RNA conformation in a plot of the major groove width against the base pair inclination angle. The major groove of the A'-RNA conformation is wide enough to accommodate a protein or peptide while that of the A-RNA conformation is too narrow to do so. The presence of the A'-RNA conformation is significant for protein-RNA interaction.

About this Structure

This structure supersedes the now removed PDB entry 373d. Full crystallographic information is available from OCA.

Reference

A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC)., Tanaka Y, Fujii S, Hiroaki H, Sakata T, Tanaka T, Uesugi S, Tomita K, Kyogoku Y, Nucleic Acids Res. 1999 Feb 15;27(4):949-55. PMID:9927725 Page seeded by OCA on Sun May 4 22:16:37 2008