Resolvase

From Proteopedia

Template:STRUCTURE 1kcf

Resolvase/Recombinase

Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination.

Holliday junction resolvase

2wiw, 2wiz, 2wj0 – AfHJR+DNA – Archaeoglobus fulgidus
2wcw, 2wcz – AfHJR (mutant)
2h8c – EcHJR (mutant) +DNA – Escherichia coli
1gdt - EcHJR +DNA
2h8e - EcHJR (mutant)
1q8r, 1hjr – EcHJR
1hjr – EcHJR RuvC
1zp7 – HJR – Bacillus subtilis
1ob8, 1hh1 – SsHJR – Sulfolobus solfataricus
2fco – HJR – Geobacillus kaustophilus
1ipi, 1gef – HJR – Pyrococcus furiosus
1kcf – HJR – fission yeast

Ser-recombinase

2rsl - EcGDR
1hx7 – EcGDR N-terminal – NMR
1ght, 1res, 1ret, 1ght – EcGDR catalytic domain – NMR
1gdr, 1hx7 - EcGDR catalytic domain
2gm4, 1zr2, 1zr4, 1gdt – EcGDR+DNA
2gm5 – EcGDR (mutant)
3guv – site-specific recombinase – Streptococcus pneumoniae
2r0q – S-rec+DNA – Staphylococcus aureus
3lhf – SsS-rec

Tyr-recombinase

3mgv, 3c28, 3c29, 2hof, 2hoi, 1xns, 1xo0, 1pvp, 1pvq, 1pvr, 1nzb, 1ouq, 1q3u, 1q3v, 1ma7, 1kbu, 1drg, 1f44, 2crx, 3crx, 4crx, 5crx, 1crx – EpCre+DNA – Enterobacteria phage PI
3etl, 3ew9, 3ewa, 2i1q – MmRadA Rec+AMPPNP+ion – Methanococcus maripaludis
2b21 - MmRadA Rec+AMPPNP
2zub, 2zuc, 2zud, 2dfl – SsRadA
2cvf, 2cvh – RadB Rec – Thermococcus kodakarensis
2f1h, 2fpl, 2fpm - MvRec+AMPPNP+K – Methanococcus voltae
2f1i – MvRec+AMPPNP
2f1j, 2fpk - MvRec+ADP
1a0p – EcXerd Rec

Integrase

2a3v – Rec INTI4+DNA – Vibrio cholerae
3bvp – Integrase N-terminal – Lactococcus phage
2oxo – LamInt binding domain – phage
1z19 – EpLamInt core binding +catalytic domain+DNA
1z1b, 1z1g, 1p7d – EpLamInt+DNA
1kjk – EpLamInt N-terminal – NMR
1ae9 – EpLamInt catalytic core
1p4e, 1m6x – yFLP (mutant)+DNA – yeast
1flo - yFLP +DNA
1aih – HP1 integrase catalytic domain - bacteriophage