Ribosomal protein S8
From Proteopedia
Ribosomal protein S8 (RPS8) is one of the 22 proteins which belong to the of the bacterial ribosome. It binds to 16S RRNA and interacts with spc operon mRNA. In archaea the protein is named S8E (RPS8E). The images at the left and at the right correspond to one representative RPS8, i.e. the crystal structure of Ribosomal protein S8 from Thermus thermophilus (2zm6, chain H).
3D Structures of Ribosomal protein S8
Update June 2012
RPS8
1an7 – TtRPS8 - Thermus thermophilus
1sei – RPS8 – Geobacillus stearothermophilus
2kcp, 2kcy – RPS8E – Methanothermobacter thermaututrophicus – NMR
2kco – RPS8E – Sulfolobus solfataricus - NMR
S8 in small ribosomal subunit
2zm6, 1n36, 1j5e, 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4, 3oto - TtRPS8 in 30S ribosomal subunit+THX+16S RRNA – Thermus thermophilus
2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq, 3pys, 3pyu - TtRPS5 in 30S ribosomal subunit+16S RRNA+RNA fragment
3kc4 – EcRPS8 in 30S ribosomal subunit - Escherichia coli
3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy, 3or9, 3ora - EcRPS8 in 30S ribosomal subunit+16S RRNA
1i94, 1fka, 3uxs, 3uxt - TtRPS8 in 30S ribosomal subunit+16S RRNA
1p87, 1p6g - EcRPS8 in 30S ribosomal subunit+16S RRNA – Cryo-EM
3bbn - RPS8 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach
S8 in small + large ribosomal subunit
1ml5 - EcRPS8 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM
S8 in small ribosomal subunit+ chain release factor
3mr8, 3ms0 – TtRPS8 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA
3d5a, 3d5c - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA
3zvo – TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Met +mRNA +16S RRNA+chain release factor 3
2b64 - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1
2b9m - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2
2x9r, 2x9t, 2wh1, 2wh3 - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA
3f1e, 3f1g - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA
3uoq - EcRPS8 + 16S RRNA + mRNA + peptide chain release factor
S8 in small ribosomal subunit+tRNA+mRNA
2hgi - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet
2hgp - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA
3t1h, 3t1y - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Lys +mRNA
3tvf, 3tvg, 3uyd, 3uyf - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Leu + tRNA fMet + mRNA
3uz6, 3uz7, 3uzg, 3uzi - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Tyr + tRNA fMet + mRNA
2i2p, 2i2u - EcRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA
2hgr - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA
1yl4 – TtRPS8 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator
1pns - EcRPS8 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA
3j0u, 3j0v, 3j0x, 3j0z, 3j10 – EcRPS8 in 30S ribosomal subunit+ A-site tRNA + P-site tRNA +16S RRNA+mRNA – Cryo-EM
3j13 – EcRPS8 in 30S ribosomal subunit+ P-site tRNA +16S RRNA+mRNA – Cryo-EM
1xnq, 1xnr - TtRPS8 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA
S8 in small ribosomal subunit+A-site tRNA
2uxd, 2uxc - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA
1n32, 1n33 - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA
1n34 - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA
1xmo, 1xmq - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA
2uxb - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA
S8 in small ribosomal subunit+P-site tRNA
3i8g - TtRPS8 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA
3i8h, 3i9b, 3i9d - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA
1ibk - TtRPS8 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA
2wwl - EcRPS8 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli
3i1q - EcRPS8 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA
3i1s, 3i1z, 3i21 - EcRPS8 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA
S8 in small ribosomal subunit+A-site tRNA+P-site tRNA
1ibl, 1ibm - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA
S8 in small ribosomal subunit+P-site tRNA+E-site tRNA
2ow8 - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA
2b9o - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA
2qnh - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA
2wdg, 2wdh, 2wdk, 2wdm - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe
S8 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA
2gy9, 2gyb - EcRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM
1jgo, 1jgp, 1jgq, 1gix - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA
3e1a, 3e1c - EcRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA
S8 in small ribosomal subunit + antibiotics
1i95, 1i97, 2hhh, 2f4v - TtRPS8 in 30S ribosomal subunit+16S RRNA+antibiotic
1hnw, 1hnx, 1hnz, 1fjg - TtRPS8 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic
2qbb, 2qb9, 1vs5, 1vs7, 2qan, 2qal, 2qou, 2qow, 2qoy, 2qp0, 2z4k, 2z4m - EcRPS8 in 30S ribosomal subunit+16S RRNA+antibiotic
2j00, 2j02 - TtRPS8 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA
3knj, 3knh - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin
3knl, 3knn - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin
3uz3, 3uz4 - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Leu + tRNA fMet + mRNA + paromomycin
3uzl, 3uzm - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Tyr + tRNA fMet + mRNA + paromomycin
S8 in small ribosomal subunit + ribosome recycling factor
2qbd, 2qbf - EcRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor
3r8n, 3r8o – EcRPS7 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA
2qbh, 2qbj - EcRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin
2v46, 2v48 - TtRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet
S8 in small ribosomal subunit + elongation factor
2wri, 2wrk, 2xsy, 2xuy - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G
2wrn, 2wrq - TtRPS8 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4
3fic - TtRPS8 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU-A
4abr - TtRPS8 in 30S ribosomal subunit+THX + E/P-site tRNA fMet +mRNA codon+16S RRNA+elongation factor TU
3izv – EcRPS8 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU
3j18 - EcRPS8 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+ mRNA + SSRA-binding protein + formyl-Met specific initiator tRNA + elongation factor G – Cryo-EM
3izw – EcRPS8 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU
2y0u, 2y0w, 2y0y, 2y10, 2y12, 2y14, 2y16, 2y18 - TtRPS8 in 30S subunit+THX+A-site tRNA+E-site tRNA+mRNA codon+16S RRNA + elongation factor TU
3fih - EcRPS8 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU
3huw, 3huy - TtRPS8 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P
2xqd - TtRPS8 in 30S ribosomal subunit+THX+E-site tRNA+mRNA +16S RRNA+elongation factor TU-B
S8 in small ribosomal subunit + translation initiation factor
1i96 - TtRPS8 in 30S ribosomal subunit+THX+IF3+16S RRNA
1hr0 - TtRPS8 in 30S ribosomal subunit+THX+IF+16S RRNA
S8 in small ribosomal subunit + various proteins
3kiq, 3kis, 3kiu, 3kix - TtRPS8 in 30S ribosomal subunit+toxin RelE+16S RRNA
1voq, 1vos, 1vov, 1vox, 1voz - EcRPS8 in 30S ribosomal subunit+protein Y+16S RRNA
2xzm, 2xzn - RPS8 in 40S subunit+18S RRNA+RACK1 - Tetrahymena thermophila
2xfz, 2xg1 - TtRPS8 in 30S ribosomal subunit+colicin E3+E-site tRNA+mRNA+16S RRNA
4dh9, 4dhb - TtRPS8 in 30S ribosomal subunit +16S RRNA+mRNA+P-site tRNA fMet +THX + YAEJ
3j00 – EcRPS8 + 16S RRNA + FTSQ + apolipoprotein A-I
2ykr – EcRPS8 + 16S RRNA + GMPPNP + GTPase RSGA
4a2i – EcRPS8 + 16S RRNA + GTPase RSGA – Cryo EM
4adv - EcRPS8 + 16S RRNA + methyltransferase – Cryo EM
3iz6 - RPS8 in 40S ribosomal subunit+RACK1 – wheat – Cryo EM
3izb - yRPS8 in 40S ribosomal subunit+ RACK1 – yeast - Cryo-EM
3u5c, 3u5g - yRPS8 in 40S ribosomal subunit+ RACK1 + suppressor protein SMT1
S8 in part of small ribosomal subunit
1qd7 – TtRPS8+TtRPS4+TtRPS6+TtRPS7+TtRPS5+TtRPS15+TtRPS17+TtRPS20+16S RRNA fragment
3iy8 - EcRPS8+EcRPS5+EcRPS6+EcRPS9+EcRPS9 EcRPS11+EcRPS12+EcRPS15+EcRPS16+EcRPS17+EcRPS18+Leishmania taretolae mRNA
1s03 - EcRPS8+spc operon mRNA
1i6u - RPS8+16S RRNA fragment – Methanococcus jannaschii
1emi - TtRPS8+16S RRNA
