User:Tilman Schirmer/Sandbox 211

From Proteopedia

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Overview

spinqualitylabelsall models Diguanylate cyclase PleD (1w25) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

from Caulobacter crescentus is a response regulator with an unorthodox catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver () domain, a Rec-like () adaptor domain, and a C-terminal domain that confers the catalytic acitvity.

The GGDEF domain is named after the highly conserved (in PleD it is GGEEF) that locates to a β-hairpin.

Substrate binding

spinqualitylabelsall models Diguanylate cyclase PleD (2v0n) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The motif is part of the as identified in the structure of PleD in complex with . The GGDEFY domain binds only one GTP subsrate molecule. For the reaction to proceed, two GTP loaded GGDEF domains have to align antiparallely.

Complete active site

(

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

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After binding of the it is believed that two GGDEF domains associate antiparallely to form a catalytically competent dimer (, ).