Invertase

From Proteopedia

spinqualitylabelsall models Invertase complex with raffinose (stick model), citrate and sulfate (PDB code 1w2t). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Invertase (INV) catalyzes the hydrolysis of sucrose into glucose and fructose[1]. INV is produced by bees which use it to make honey from nectar. Cell-wall INV is crucial for metabolism, growth and differentiation in plants[2]. Extracellular INV catalyzes the cleavage of the transport sugar sucrose released into the app-last[3] Alkaline INV is a tetramer which catalyzes the cleavage of sucrose[4] DNA INV Gin is found in bacteriophage Mu and catalyses the recombination between repeat sequences in vivo and in vitro[5] For details in Hebrew see Invertase (Hebrew). Relevance INV is used in the food industry to produce fructose which is sweeter and does not crystallize easily. Structural highlights The of INV contains the catalytic residues Asp and Glu[6].

3D Structures of invertase

Updated on 07-July-2024

References

1. ↑ Neumann NP, Lampen JO. Purification and properties of yeast invertase. Biochemistry. 1967 Feb;6(2):468-75. PMID:4963242
2. ↑ Proels RK, Huckelhoven R. Cell-wall invertases, key enzymes in the modulation of plant metabolism during defence responses. Mol Plant Pathol. 2014 Oct;15(8):858-64. doi: 10.1111/mpp.12139. Epub 2014 May, 13. PMID:24646208 doi:http://dx.doi.org/10.1111/mpp.12139
3. ↑ Roitsch T, Balibrea ME, Hofmann M, Proels R, Sinha AK. Extracellular invertase: key metabolic enzyme and PR protein. J Exp Bot. 2003 Jan;54(382):513-24. PMID:12508062
4. ↑ Lee HS, Sturm A. Purification and characterization of neutral and alkaline invertase from carrot. Plant Physiol. 1996 Dec;112(4):1513-22. PMID:8972597 doi:10.1104/pp.112.4.1513
5. ↑ Klippel A, Mertens G, Patschinsky T, Kahmann R. The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9. EMBO J. 1988 Apr;7(4):1229-37. PMID:3042382 doi:10.1002/j.1460-2075.1988.tb02935.x
6. ↑ Alberto F, Jordi E, Henrissat B, Czjzek M. Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose. Biochem J. 2006 May 1;395(3):457-62. PMID:16411890 doi:http://dx.doi.org/BJ20051936