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1s8f

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Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II

Structural highlights

1s8f is a 2 chain structure with sequence from Canis lupus familiaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.77Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAB9A_CANLF Involved in the transport of proteins between the endosomes and the trans-Golgi network (PubMed:8440258). Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).[UniProtKB:Q9R0M6]^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The small GTPase Rab9 is an essential regulator of vesicular transport from the late endosome to the trans-Golgi network, as monitored by the redirection of the mannose-6-phosphate receptors. The crystal structure of Rab9 complexed to GDP, Mg(2+), and Sr(2+) reveals a unique dimer formed by an intermolecular beta-sheet that buries the switch I regions. Surface area and shape complementarity calculations suggest that Rab9 dimers can form an inactive, membrane-bound pool of Rab9 . GDP that is independent of GDI. Mg(2+)-bound Rab9 represents an inactive state, but Sr(2+)-bound Rab9 . GDP displays activated switch region conformations, mimicking those of the GTP state. A hydrophobic tetrad is formed resembling an effector-discriminating epitope found only in GTP-bound Rab proteins.

Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II.,Wittmann JG, Rudolph MG FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:15196914^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lombardi D, Soldati T, Riederer MA, Goda Y, Zerial M, Pfeffer SR. Rab9 functions in transport between late endosomes and the trans Golgi network. EMBO J. 1993 Feb;12(2):677-82. PMID:8440258 doi:10.1002/j.1460-2075.1993.tb05701.x
↑ Wittmann JG, Rudolph MG. Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II. FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:15196914 doi:10.1016/j.febslet.2004.05.004