Structural highlights
Function
APTX_SCHPO DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).
Publication Abstract from PubMed
Aprataxin is a DNA deadenylase that resolves DNA 5'-AMP termini and reverses abortive DNA ligation. The crystal structures of Schizosaccharomyces pombe aprataxin Hnt3 in its apo form and in complex to dsDNA and dsDNA-AMP reveal how Hnt3 recognizes and processes 5'-adenylated DNA in a structure-specific manner. The bound DNA adopts a 5'-flap conformation that facilitates 5'-AMP access to the active site, where AMP cleavage occurs by a canonical catalytic mechanism.
Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA.,Gong Y, Zhu D, Ding J, Dou CN, Ren X, Gu L, Jiang T, Wang DC Nat Struct Mol Biol. 2011 Oct 9;18(11):1297-9. doi: 10.1038/nsmb.2145. PMID:21984208[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gong Y, Zhu D, Ding J, Dou CN, Ren X, Gu L, Jiang T, Wang DC. Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA. Nat Struct Mol Biol. 2011 Oct 9;18(11):1297-9. doi: 10.1038/nsmb.2145. PMID:21984208 doi:10.1038/nsmb.2145
