7bwk
From Proteopedia
Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila
Structural highlights
FunctionDOTL_LEGPH Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). Plays a central role in the assembly of the subcomplex (PubMed:32513920). Required for the recruitment of IcmS and IcmW to the inner membrane and for the translocation of adapter-dependent substrates (PubMed:23028312). May have ATPase activity (Probable).[1] [2] [3] [4] [5] Publication Abstract from PubMedThe Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) is extremely versatile, translocating ~300 effector proteins into host cells. This specialized secretion system employs the Dot/Icm type IVB coupling protein (T4CP) complex, which includes IcmS, IcmW and LvgA, that are known to selectively assist the export of a subclass of effectors. Herein, the crystal structure of a four-subunit T4CP subcomplex bound to the effector protein VpdB reveals an interaction between LvgA and a linear motif in the C-terminus of VpdB. The same binding interface of LvgA also interacts with the C-terminal region of three additional effectors, SidH, SetA and PieA. Mutational analyses identified a FxxxLxxxK binding motif that is shared by VpdB and SidH, but not by SetA and PieA, showing that LvgA recognizes more than one type of binding motif. Together, this work provides a structural basis for how the Dot/Icm T4CP complex recognizes effectors, and highlights the multiple substrate-binding specificities of its adaptor subunit. Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex.,Kim H, Kubori T, Yamazaki K, Kwak MJ, Park SY, Nagai H, Vogel JP, Oh BH Nat Commun. 2020 May 26;11(1):2623. doi: 10.1038/s41467-020-16397-0. PMID:32457311[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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