Structural highlights
Function
U2AF1_SCHPO Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.[1]
Publication Abstract from PubMed
The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3 splice site, which includes an essential AG dinucleotide. Even a single amino acid mutation of U2AF1 can cause serious disease such as certain cancers or myelodysplastic syndromes. Here, we describe the first crystal structures of wild-type and pathogenic mutant U2AF1 complexed with target RNA, revealing the mechanism of 3 splice site selection, and how aberrant splicing results from clinically important mutations. Unexpected features of this mechanism may assist the future development of new treatments against diseases caused by splicing errors.
Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.,Yoshida H, Park SY, Sakashita G, Nariai Y, Kuwasako K, Muto Y, Urano T, Obayashi E Nat Commun. 2020 Sep 21;11(1):4744. doi: 10.1038/s41467-020-18559-6. PMID:32958768[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Huang T, Vilardell J, Query CC. Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23). EMBO J. 2002 Oct 15;21(20):5516-26. PMID:12374752
- ↑ Yoshida H, Park SY, Sakashita G, Nariai Y, Kuwasako K, Muto Y, Urano T, Obayashi E. Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1. Nat Commun. 2020 Sep 21;11(1):4744. PMID:32958768 doi:10.1038/s41467-020-18559-6
