8bv8

Structural highlights

Function

MOM_BPMU Iron-binding protein that performs methylcarbamoylation of adenine using acetyl CoA (PubMed:32369169). This chemical modificaltion makes the viral DNA resistant to a variety of host type I and type II restriction enzymes by modifying approximately 15% of DNA adenine residues (PubMed:32369169). The modification called momylation changes adenine for N6-methylcarbamoyl adenine and occurs just before packaging (PubMed:1258376, PubMed:32369169). Target sequences are 5'-(C or G)-A-(Cor G)-N-(C or T)-3' (PubMed:1258376). Also usually modifies adenine residues in the host cellular DNA (PubMed:1258376).^{[1] [2]}

References

1. ↑ Toussaint A. The DNA modification function of temperate phage Mu-1. Virology. 1976 Mar;70(1):17-27. PMID:1258376 doi:10.1016/0042-6822(76)90232-4
2. ↑ Karambelkar S, Udupa S, Gowthami VN, Ramachandra SG, Swapna G, Nagaraja V. Emergence of a novel immune-evasion strategy from an ancestral protein fold in bacteriophage Mu. Nucleic Acids Res. 2020 Jun 4;48(10):5294-5305. PMID:32369169 doi:10.1093/nar/gkaa319