Structural highlights
8odw is a 2 chain structure with sequence from Gynuella sunshinyii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 3.07Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A0C5VQJ2_9GAMM
Publication Abstract from PubMed
Modular trans-acyltransferase polyketide synthases (trans-AT PKSs) are enzymatic assembly lines that biosynthesize complex polyketide natural products. Relative to their better studied cis-AT counterparts, the trans-AT PKSs introduce remarkable chemical diversity into their polyketide products. A notable example is the lobatamide A PKS, which incorporates a methylated oxime. Here we demonstrate biochemically that this functionality is installed on-line by an unusual oxygenase-containing bimodule. Furthermore, analysis of the oxygenase crystal structure coupled with site-directed mutagenesis allows us to propose a model for catalysis, as well as identifying key protein-protein interactions that support this chemistry. Overall, our work adds oxime-forming machinery to the biomolecular toolbox available for trans-AT PKS engineering, opening the way to introducing such masked aldehyde functionalities into diverse polyketides.
Modular oxime formation by a trans-AT polyketide synthase.,Minas HA, Francois RMM, Hemmerling F, Fraley AE, Dieterich CL, Rudisser SH, Meoded RA, Collin S, Weissman KJ, Gruez A, Piel J Angew Chem Int Ed Engl. 2023 May 22:e202304481. doi: 10.1002/anie.202304481. PMID:37216334[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Minas HA, François RMM, Hemmerling F, Fraley AE, Dieterich CL, Rüdisser SH, Meoded RA, Collin S, Weissman KJ, Gruez A, Piel J. Modular oxime formation by a trans-AT polyketide synthase. Angew Chem Int Ed Engl. 2023 May 22:e202304481. PMID:37216334 doi:10.1002/anie.202304481
