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<StructureSection load='1mnm' size='350' side='right' caption='YEAST MATALPHA2/MCM1/DNA TERNARY TRANSCRIPTION COMPLEX CRYSTAL STRUCTURE (PDB entry [[1mnm]])' scene=''> The structure of a complex containing the homeodomain repressor protein MATalpha2 and the MADS-box transcription factor '''MCM1''' bound to DNA has been determined by X-ray crystallography at 2.25 A resolution. [[MtrF]] is a cell surface cytochrome on the Gram-negative bacteria known as ''Shewanella oneidensis''.<ref name="mtrf"> PMID:11418600</ref> MtrF is involved with shuttling electrons across its (''S. oneidensis'') outer surface. MtrF has several homologues, MtrC and the protein OmcA. These three different proteins are thought to be replaceable with one another in deletion mutation experiments.<ref name= "mtrf" /> __TOC__ [[Image:MW_Folding_Simulations.gif]] <scene name='Sandbox_UC_11/Escena1/3'> Secuencia desde N a C terminal </scene> <scene name='Sandbox_UC_11/Structura_secundaria/1'>TextToBeDisplayed</scene> ==Structure== Through the structure of this oligomer that was obtained by crystallography, we can see that HDAC9 is kept <scene name='Sandbox_Reserved_1/Binding/3'>relatively far from the genomic DNA </scene> even after MEF2 binding to the chromosome. We can also easily observe a very well <scene name='Sandbox_Reserved_1/Conservation/7'>evolutionary conserved region</scene> near the DNA binding site of MEF2. {{Template:ColorKey_Amino2CarboxyRainbow}} </StructureSection> ==Directed evolution and Colicin7/Immunity-proteins complexes<ref>PMID:19749752</ref>== <StructureSection load='3gkl' size='500' frame='true' align='right' scene='3gkl/Al/1' > {{Clear}} <scene name='3gkl/Align/2'>Structural alignment</scene> of the immunity protein 9 (Im9, [[1bxi]], <span style="color:yellow;background-color:black;font-weight:bold;">colored yellow</span>), <span style="color:lime;background-color:black;font-weight:bold;">evolved variant R12-2 (lime)</span>, and <font color='blue'><b>immunity protein 7 (Im7, colored blue</b></font>, [[7cei]]) reveals their structural identity. However, when the immunity proteins-bound <scene name='3gkl/Align/3'>colicins within their complexes were aligned</scene>, they demonstrate somewhat different picture. {{Clear}} <quiz display= simple> {¿Cuántas hélices alfa se encuentran en esta estructura?| Type= "[]"} - Ninguno + One - Cuatro </ Quiz> MCM1 bound to DNA <ref> '''MCM1''' bound to DNA </ref> </StructureSection> <references/> {{STRUCTURE_1mnm}} ==Additional Literature== <ref group="xtra">PMID:010074943</ref> <ref group='xtra'>PMID:19749752</ref> <references group="xtra"/> [[Category: Escherichia coli]] [[Category: Hemmings, A M.]] [[Category: James, R.]] [[Category: Kuhlmann, U C.]] [[Category: Moore, G R.]] [[Category: Colicin]] [[Category: Endonuclease]] [[Category: Immune system]] [[Category: Protein-protein interaction]] ==External Resources== * [http://news.yahoo.com/s/ap/20100911/ap_on_re_us/us_fireflies_military_research Yahoo news coverage of luciferase studies funded research for military applications] * [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia] * [http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase: June 2006 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by *[[Lipase]], the main article in Proteopedia. *[[Molecular Playground/Pancreatic Lipase]] *[http://en.wikipedia.org/wiki/Lipase Lipase in Wikipedia]

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