1e4o
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1E4O is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ | + | 1E4O is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phosphorylase Phosphorylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.1 2.4.1.1]]. Structure known Active Sites: PLA and PLB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E4O OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: phosphorylase mechanism]] | [[Category: phosphorylase mechanism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 09: | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 09:56:34 2007'' |
Revision as of 07:51, 30 October 2007
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PHOSPHORYLASE RECOGNITION AND PHOSPHOROLYSIS OF ITS OLIGOSACCHARIDE SUBSTRATE: ANSWERS TO A LONG OUTSTANDING QUESTION
Overview
Phosphorylases are key enzymes of carbohydrate metabolism. Structural, studies have provided explanations for almost all features of control and, substrate recognition of phosphorylase but one question remains, unanswered. How does phosphorylase recognize and cleave an oligosaccharide, substrate? To answer this question we turned to the Escherichia coli, maltodextrin phosphorylase (MalP), a non-regulatory phosphorylase that, shares similar kinetic and catalytic properties with the mammalian, glycogen phosphorylase. The crystal structures of three, MalP-oligosaccharide complexes are reported: the binary complex of MalP, with the natural substrate, maltopentaose (G5); the binary complex with, the thio-oligosaccharide, 4-S-alpha-D-glucopyranosyl-4-thiomaltotetraose, (GSG4), both at 2.9 A ... [(full description)]
About this Structure
1E4O is a [Single protein] structure of sequence from [Escherichia coli] with PLP as [ligand]. Active as [Phosphorylase], with EC number [2.4.1.1]. Structure known Active Sites: PLA and PLB. Full crystallographic information is available from [OCA].
Reference
Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: answers to a long outstanding question., Watson KA, McCleverty C, Geremia S, Cottaz S, Driguez H, Johnson LN, EMBO J. 1999 Sep 1;18(17):4619-32. PMID:10469642
Page seeded by OCA on Tue Oct 30 09:56:34 2007
