Anthony Noles Sandbox
From Proteopedia
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| - | =Aconitase= (PDB [[2b3x]]) catalyzes the reversible isomerization of citrate and isocitrate. | + | =Aconitase= |
| + | Aconitase (PDB [[2b3x]]) catalyzes the reversible isomerization of citrate and isocitrate. | ||
==Mechanism of Aconitase== | ==Mechanism of Aconitase== | ||
{{STRUCTURE_2b3x | PDB=2b3x | SCENE= }} | {{STRUCTURE_2b3x | PDB=2b3x | SCENE= }} | ||
====Stage 1: Dehydration==== | ====Stage 1: Dehydration==== | ||
| - | + | First, dehydration of citrate causes a proton and OH group to be removed from only the 'lower arm'.<ref>Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. p. 578. Print.</ref> This forms a cis-Aconitate intermediate. | |
Show mechanism here! | Show mechanism here! | ||
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| - | + | ==References== | |
<references/> | <references/> | ||
| - | + | ==External Links== | |
[http://en.wikipedia.org/wiki/Aconitase Aconitase on Wikipedia] | [http://en.wikipedia.org/wiki/Aconitase Aconitase on Wikipedia] | ||
Revision as of 01:28, 27 February 2010
Contents |
Aconitase
Aconitase (PDB 2b3x) catalyzes the reversible isomerization of citrate and isocitrate.
Mechanism of Aconitase
Stage 1: Dehydration
First, dehydration of citrate causes a proton and OH group to be removed from only the 'lower arm'.[1] This forms a cis-Aconitate intermediate.
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Aconitase contains 4Fe-4S iron-sulfur cluster. This iron sulfur cluster does not participate in redox as most do, but coordinates the OH goup of citrate to facilitate its elimination.[2]
Stage 2: Rehydration
The second main stage of the reaction is the rehydration of the cis-Aconitate intermediate. This forms isocitrate. It is catalyzed in a stereospecific way such that only one isocitrate stereoisomer is formed. [3]
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Thus, the overall reaction that aconitase catalyzes is:
Show total mechanism here!
References
- ↑ Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. p. 578. Print.
- ↑ Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC. Crystal structure of human iron regulatory protein 1 as cytosolic aconitase. Structure. 2006 Jan;14(1):129-39. PMID:16407072 doi:10.1016/j.str.2005.09.009
- ↑ Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry Life at the Molecular Level. New York: John Wiley & Sons, 2008. p. 579. Print.
External Links
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