1e9m
From Proteopedia
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| - | [[Image:1e9m. | + | [[Image:1e9m.jpg|left|200px]]<br /><applet load="1e9m" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e9m" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1e9m, resolution 2.07Å" /> | caption="1e9m, resolution 2.07Å" /> | ||
'''FERREDOXIN VI FROM RHODOBACTER CAPSULATUS'''<br /> | '''FERREDOXIN VI FROM RHODOBACTER CAPSULATUS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus] with FES as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1E9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus] with FES as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=FES:Binding Site For Fes (Cluster 2 Irons-2 Sulfurs)'>FES</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ferredoxin]] | [[Category: ferredoxin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:06:13 2007'' |
Revision as of 12:56, 18 December 2007
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FERREDOXIN VI FROM RHODOBACTER CAPSULATUS
Overview
A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction analysis, indicated that FdVI crystals are orthorhombic and belong to the space, group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c =, 54.29 A.
About this Structure
1E9M is a Single protein structure of sequence from Rhodobacter capsulatus with FES as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus., Armengaud J, Sainz G, Jouanneau Y, Sieker LC, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):301-3. PMID:11173487[[Category: [2fe-2s]]]
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