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| - | [[Image:1adt.gif|left|200px]]<br /><applet load="1adt" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[2wb0]] This PDB entry is obsolete and replaced by 2wb0 |
| - | caption="1adt, resolution 2.6Å" />
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| - | '''EARLY E2A DNA-BINDING PROTEIN'''<br />
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| - | ==Overview==
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| - | The adenovirus single-stranded DNA binding protein (Ad DBP) is a multifunctional protein required, amongst other things, for DNA replication and transcription control. It binds to single- and double-stranded DNA, as well as to RNA, in a sequence-independent manner. Like other single-stranded DNA binding proteins, it binds ssDNA, cooperatively. We report the crystal structure, at 2.6 A resolution, of the nucleic acid binding domain. This domain is active in DNA replication. The protein contains two zinc atoms in different, novel coordinations. The zinc atoms appear to be required for the stability of the protein fold rather than being involved in direct contacts with the DNA. The crystal structure shows that the protein contains a 17 amino acid C-terminal extension which hooks onto a second molecule, thereby forming a protein chain. Deletion of this C-terminal arm reduces cooperativity in DNA binding, suggesting a hook-on model for cooperativity. Based on this structural work and mutant studies, we propose that DBP forms a protein core around which the single-stranded DNA winds.
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| - | ==About this Structure==
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| - | 1ADT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_11 Human adenovirus 11] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ADT OCA].
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| - | ==Reference==
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| - | Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding., Tucker PA, Tsernoglou D, Tucker AD, Coenjaerts FE, Leenders H, van der Vliet PC, EMBO J. 1994 Jul 1;13(13):2994-3002. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8039495 8039495]
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| - | [[Category: Human adenovirus 11]]
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| - | [[Category: Single protein]]
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| - | [[Category: Coenjaerts, F E.J.]]
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| - | [[Category: Leenders, H.]]
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| - | [[Category: Tsernoglou, D.]]
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| - | [[Category: Tucker, A D.]]
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| - | [[Category: Tucker, P A.]]
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| - | [[Category: Vliet, P C.Van Der.]]
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| - | [[Category: ZN]]
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| - | [[Category: dna-binding protein]]
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| - | [[Category: ssdna binding protein]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:23 2008''
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