1h7k
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1H7K is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]] with ACT, SO4 and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | + | 1H7K is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]] with ACT, SO4 and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Catalase Catalase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H7K OCA]]. |
==Reference== | ==Reference== | ||
Formation of a tyrosyl radical intermediate in Proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity., Andreoletti P, Gambarelli S, Sainz G, Stojanoff V, White C, Desfonds G, Gagnon J, Gaillard J, Jouve HM, Biochemistry. 2001 Nov 13;40(45):13734-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11695923 11695923] | Formation of a tyrosyl radical intermediate in Proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity., Andreoletti P, Gambarelli S, Sainz G, Stojanoff V, White C, Desfonds G, Gagnon J, Gaillard J, Jouve HM, Biochemistry. 2001 Nov 13;40(45):13734-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11695923 11695923] | ||
| + | [[Category: Catalase]] | ||
[[Category: Proteus mirabilis]] | [[Category: Proteus mirabilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: peroxidase]] | [[Category: peroxidase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:28:59 2007'' |
Revision as of 08:24, 30 October 2007
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FORMATION OF A TYROSYL RADICAL INTERMEDIATE IN PROTEUS MIRABILIS CATALASE BY DIRECTED MUTAGENESIS AND CONSEQUENCES FOR NUCLEOTIDE REACTIVITY
Overview
Proteus mirabilis catalase (PMC) belongs to the family of NADPH binding, catalases. The function of NADPH in these enzymes is still a matter of, debate. This study presents the effects of two independent phenylalanine, mutations (F194 and F215), located between NADPH and heme in the PMC, structure. The phenylalanines were replaced with tyrosines which we, predicted could carry radicals in a NADPH-heme electron transfer. The, X-ray crystal structures of the two mutants indicated that neither the, binding site of NADPH nor the immediate environment of the residues was, affected by the mutations. Measurements using H2O2 as a substrate, confirmed that the variants were as active as the native enzyme. With, equivalent amounts of peroxoacetic acid, wild-type PMC, F215Y PMC, and, beef liver ... [(full description)]
About this Structure
1H7K is a [Single protein] structure of sequence from [Proteus mirabilis] with ACT, SO4 and HEM as [ligands]. Active as [Catalase], with EC number [1.11.1.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Formation of a tyrosyl radical intermediate in Proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity., Andreoletti P, Gambarelli S, Sainz G, Stojanoff V, White C, Desfonds G, Gagnon J, Gaillard J, Jouve HM, Biochemistry. 2001 Nov 13;40(45):13734-43. PMID:11695923
Page seeded by OCA on Tue Oct 30 10:28:59 2007
Categories: Catalase | Proteus mirabilis | Single protein | Andreoletti, P. | Gaillard, J. | Gambarelli, S. | Jouve, H.M. | Sainz, G. | Stojanoff, V. | ACT | HEM | SO4 | Hem | Hydrogen peroxide | Iron | Nadp | Oxidoreductase (h2o2 acceptor) | Peroxidase
