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1gwa
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1GWA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA, SO4 and IOD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | + | 1GWA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA, SO4 and IOD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]]. Structure known Active Site: CAA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GWA OCA]]. |
==Reference== | ==Reference== | ||
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037300 12037300] | Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037300 12037300] | ||
| + | [[Category: Pancreatic elastase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:31:46 2007'' |
Revision as of 08:27, 30 October 2007
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TRIIODIDE DERIVATIVE OF PORCINE PANCREAS ELASTASE
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house, X-ray equipment are presented which investigate two possibilities for, enhancing the experimental phasing signal by means of (i) triiodide/iodide, soaks using KI/I(2) and (ii) combinations of counter-ions introduced using, the short cryosoak method. Triiodide-derivative crystal structures for, five test proteins have been refined and reveal that iodine can bind as, polyiodide and single iodide ions through hydrophobic and hydrogen-bonding, interactions both at the molecular surface and in intramolecular and, intermolecular cavities. In three cases, the structures could be, automatically determined with autoSHARP using in-house SAD and SIRAS data., The investigation of combinatorial counter-ion replacement using ... [(full description)]
About this Structure
1GWA is a [Single protein] structure of sequence from [Sus scrofa] with CA, SO4 and IOD as [ligands]. Active as [Pancreatic elastase], with EC number [3.4.21.36]. Structure known Active Site: CAA. Full crystallographic information is available from [OCA].
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300
Page seeded by OCA on Tue Oct 30 10:31:46 2007
Categories: Pancreatic elastase | Single protein | Sus scrofa | Bricogne, G. | Evans, G. | CA | IOD | SO4 | Hydrolase | Serine protease | Signal | Zymogen
