1of3
From Proteopedia
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| - | [[Image:1of3. | + | [[Image:1of3.jpg|left|200px]]<br /><applet load="1of3" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1of3" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1of3, resolution 2.00Å" /> | caption="1of3, resolution 2.00Å" /> | ||
'''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE, TMCBM27'''<br /> | '''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE, TMCBM27'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OF3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1OF3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OF3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tmcbm27]] | [[Category: tmcbm27]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:39:41 2007'' |
Revision as of 15:29, 18 December 2007
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STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE, TMCBM27
Overview
The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5), constitute a carbohydrate binding module (CBM) that has been classified, into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds, tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose, (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal, structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a, TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6, A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for, mannans. In particular, the latter complex, which is the first structure, of a CBM in complex with a branched plant cell wall polysaccharide, illustrates how the architecture of the binding site can influence the, recognition of naturally substituted polysaccharides.
About this Structure
1OF3 is a Single protein structure of sequence from Thermotoga maritima with CA as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:12791255
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