1uns
From Proteopedia
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| - | [[Image:1uns. | + | [[Image:1uns.jpg|left|200px]]<br /><applet load="1uns" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1uns" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1uns, resolution 2.0Å" /> | caption="1uns, resolution 2.0Å" /> | ||
'''IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION'''<br /> | '''IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1UNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UNS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc-binding site]] | [[Category: zinc-binding site]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:04:43 2007'' |
Revision as of 15:54, 18 December 2007
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IDENTIFICATION OF A SECONDARY ZINC-BINDING SITE IN STAPHYLOCOCCAL ENTEROTOXIN C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION
Overview
The previously determined crystal structure of the superantigen, staphylococcal enterotoxin C2 (SEC2) showed binding of a single zinc ion, located between the N- and C-terminal domains. Here we present the crystal, structure of SEC2 determined to 2.0 A resolution in the presence of, additional zinc. The structure revealed the presence of a secondary, zinc-binding site close to the major histocompatibility complex, (MHC)-binding site of the toxin and some 28 A away from the primary, zinc-binding site of the toxin found in previous studies. T cell, stimulation assays showed that varying the concentration of zinc ions, present affected the activity of the toxin and we observed that high zinc, concentrations considerably inhibited T cell responses. This indicates, that SEC2 may have multiple modes of interaction with the immune system, that are dependent on serum zinc levels. The potential role of the, secondary zinc-binding site and that of the primary one in the formation, of the TCR.SEC2.MHC complex are considered, and the possibility that zinc, may regulate the activity of SEC2 as a toxin facilitating different T cell, responses is discussed.
About this Structure
1UNS is a Single protein structure of sequence from Staphylococcus aureus with ZN as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Identification of a secondary zinc-binding site in staphylococcal enterotoxin C2. Implications for superantigen recognition., Papageorgiou AC, Baker MD, McLeod JD, Goda SK, Manzotti CN, Sansom DM, Tranter HS, Acharya KR, J Biol Chem. 2004 Jan 9;279(2):1297-303. Epub 2003 Oct 14. PMID:14559915
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