1w0n

From Proteopedia

Revision as of 16:15, 18 December 2007

STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36

Overview

The enzymatic degradation of polysaccharides harnesses multimodular, enzymes whose carbohydrate binding modules (CBM) target the catalytic, domain onto the recalcitrant substrate. Here we report the ab initio, structure determination and subsequent refinement, at 0.8 A resolution, of, the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity, electrophoresis, isothermal titration calorimetry, and UV difference, spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan, binding domain. The 3D structure of CBM36 in complex with xylotriose and, Ca(2+), at 1.5 A resolution, displays significant conformational changes, compared to the native structure and reveals the molecular basis for its, unique Ca(2+)-dependent binding of xylooligosaccharides through, coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging, spectrum of carbohydrate binding modules that increasingly find, applications in industry and display great potential for mapping the, "glyco-architecture" of plant cells.

About this Structure

1W0N is a Single protein structure of sequence from Paenibacillus polymyxa with CA, MG and SO4 as ligands. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:15242594

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