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1w16
From Proteopedia
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| - | [[Image:1w16.gif|left|200px]]<br /> | + | [[Image:1w16.gif|left|200px]]<br /><applet load="1w16" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w16" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w16, resolution 2.30Å" /> | caption="1w16, resolution 2.30Å" /> | ||
'''RAT SYNAPTOTAGMIN 4 C2B DOMAIN IN THE ABSENCE OF CALCIUM'''<br /> | '''RAT SYNAPTOTAGMIN 4 C2B DOMAIN IN THE ABSENCE OF CALCIUM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1W16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W16 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:25:55 2007'' |
Revision as of 16:16, 18 December 2007
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RAT SYNAPTOTAGMIN 4 C2B DOMAIN IN THE ABSENCE OF CALCIUM
Overview
The neuronal protein synaptotagmin 1 functions as a Ca(2+) sensor in, exocytosis via two Ca(2+)-binding C(2) domains. The very similar, synaptotagmin 4, which includes all the predicted Ca(2+)-binding residues, in the C(2)B domain but not in the C(2)A domain, is also thought to, function as a neuronal Ca(2+) sensor. Here we show that, unexpectedly, both C(2) domains of fly synaptotagmin 4 exhibit Ca(2+)-dependent, phospholipid binding, whereas neither C(2) domain of rat synaptotagmin 4, binds Ca(2+) or phospholipids efficiently. Crystallography reveals that, changes in the orientations of critical Ca(2+) ligands, and perhaps their, flexibility, render the rat synaptotagmin 4 C(2)B domain unable to form, full Ca(2+)-binding sites. These results indicate that synaptotagmin 4 is, a Ca(2+) sensor in the fly but not in the rat, that the Ca(2+)-binding, properties of C(2) domains cannot be reliably predicted from sequence, analyses, and that proteins clearly identified as orthologs may, nevertheless have markedly different functional properties.
About this Structure
1W16 is a Single protein structure of sequence from Rattus norvegicus with NA and CL as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4., Dai H, Shin OH, Machius M, Tomchick DR, Sudhof TC, Rizo J, Nat Struct Mol Biol. 2004 Sep;11(9):844-9. Epub 2004 Aug 15. PMID:15311271
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