This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1w3r
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1w3r. | + | [[Image:1w3r.jpg|left|200px]]<br /><applet load="1w3r" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w3r" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w3r, resolution 1.90Å" /> | caption="1w3r, resolution 1.90Å" /> | ||
'''NIMA FROM D. RADIODURANS WITH METRONIDAZOLE AND PYRUVATE'''<br /> | '''NIMA FROM D. RADIODURANS WITH METRONIDAZOLE AND PYRUVATE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1W3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with ACT, PYR and 2MN as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1W3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with ACT, PYR and 2MN as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:2mn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W3R OCA]. |
==Reference== | ==Reference== | ||
| Line 29: | Line 28: | ||
[[Category: nim gene]] | [[Category: nim gene]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:29:24 2007'' |
Revision as of 16:19, 18 December 2007
|
NIMA FROM D. RADIODURANS WITH METRONIDAZOLE AND PYRUVATE
Overview
5-Nitroimidazole-based antibiotics are compounds extensively used for, treating infections in humans and animals caused by several important, pathogens. They are administered as prodrugs, and their activation depends, upon an anaerobic 1-electron reduction of the nitro group by a reduction, pathway in the cells. Bacterial resistance toward these drugs is thought, to be caused by decreased drug uptake and/or an altered reduction, efficiency. One class of resistant strains, identified in Bacteroides, has, been shown to carry Nim genes (NimA, -B, -C, -D, and -E), which encode for, reductases that convert the nitro group on the antibiotic into a, non-bactericidal amine. In this paper, we have described the crystal, structure of NimA from Deinococcus radiodurans (drNimA) at 1.6 A, resolution. We have shown that drNimA is a homodimer in which each monomer, adopts a beta-barrel fold. We have identified the catalytically important, His-71 along with the cofactor pyruvate and antibiotic binding sites, all, of which are found at the monomer-monomer interface. We have reported, three additional crystal structures of drNimA, one in which the antibiotic, metronidazole is bound to the protein, one with pyruvate covalently bound, to His-71, and one with lactate covalently bound to His-71. Based on these, structures, a reaction mechanism has been proposed in which the 2-electron, reduction of the antibiotic prevents accumulation of the toxic nitro, radical. This mechanism suggests that Nim proteins form a new class of, reductases, conferring resistance against 5-nitroimidazole-based, antibiotics.
About this Structure
1W3R is a Single protein structure of sequence from Deinococcus radiodurans with ACT, PYR and 2MN as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of 5-nitroimidazole antibiotic resistance: the crystal structure of NimA from Deinococcus radiodurans., Leiros HK, Kozielski-Stuhrmann S, Kapp U, Terradot L, Leonard GA, McSweeney SM, J Biol Chem. 2004 Dec 31;279(53):55840-9. Epub 2004 Oct 18. PMID:15492014
Page seeded by OCA on Tue Dec 18 18:29:24 2007
