1w9x
From Proteopedia
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| - | [[Image:1w9x.gif|left|200px]]<br /> | + | [[Image:1w9x.gif|left|200px]]<br /><applet load="1w9x" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w9x" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w9x, resolution 2.10Å" /> | caption="1w9x, resolution 2.10Å" /> | ||
'''BACILLUS HALMAPALUS ALPHA AMYLASE'''<br /> | '''BACILLUS HALMAPALUS ALPHA AMYLASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W9X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_halmapalus Bacillus halmapalus] with CA and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] | + | 1W9X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_halmapalus Bacillus halmapalus] with CA and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Ac1 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W9X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:35:47 2007'' |
Revision as of 16:25, 18 December 2007
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BACILLUS HALMAPALUS ALPHA AMYLASE
Overview
The enzymatic digestion of starch by alpha-amylases is one of the key, biotechnological reactions of recent times. In the search for industrial, biocatalysts, the family GH13 alpha-amylase BHA from Bacillus halmapalus, has been cloned and expressed. The three-dimensional structure at 2.1 A, resolution has been determined in complex with the (pseudo)tetrasaccharide, inhibitor acarbose. Acarbose is found bound as a nonasaccharide, transglycosylation product spanning the -6 to +3 subsites. Careful, inspection of electron density suggests that the bound ligand could not, have been formed through successive transglycosylations of acarbose and, must also have featured maltose or maltooligosaccharides as an acceptor.
About this Structure
1W9X is a Single protein structure of sequence from Bacillus halmapalus with CA and NA as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a Bacillus halmapalus family 13 alpha-amylase, BHA, in complex with an acarbose-derived nonasaccharide at 2.1 A resolution., Davies GJ, Brzozowski AM, Dauter Z, Rasmussen MD, Borchert TV, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):190-3. Epub 2005, Jan 19. PMID:15681870
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