1wc7
From Proteopedia
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| - | [[Image:1wc7.gif|left|200px]]<br /> | + | [[Image:1wc7.gif|left|200px]]<br /><applet load="1wc7" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1wc7" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1wc7, resolution 2.33Å" /> | caption="1wc7, resolution 2.33Å" /> | ||
'''FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE'''<br /> | '''FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with IOD and PP3 as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1WC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with IOD and PP3 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Iod Binding Site For Chain L'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WC7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transamination]] | [[Category: transamination]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:38:09 2007'' |
Revision as of 16:28, 18 December 2007
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FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE
Overview
The purification, crystallization and structure determination at 2.3 A, resolution of the complex of the pyridoxal-5'-phosphate (PLP) dependent, catalytic antibody 15A9 with a phosphopyridoxyl-L-alanine (PPL-L-alanine), substrate analogue are described. The crystal belongs to space group, P2(1), with two molecules in the asymmetric unit related by, non-crystallographic symmetry. The unit-cell parameters are a = 63.5, b =, 81.7, c = 79.3 A and beta is fortuitously 90 degrees . Refinement of the, structure converged at unacceptably high R factors. Although the, traditional analysis of intensity distribution did not indicate twinning, pseudomerohedral twinning was revealed by a newer test based on local, intensity differences [Padilla & Yeates (2003), Acta Cryst. D59, 1124-1130]. When the potential twinning operator was included in SHELX, the structure could be satisfactorily refined with a twinning fraction of, 0.46, indicating a nearly perfect hemihedrally twinned crystal. One of the, active sites is occupied by the phosphopyridoxyl-L-alanine ligand, while, one iodide ion mimics the cofactor phosphate group in the other. Four, other iodide ions are present in the structure: two are involved in, specific intermolecular contacts and two dictate the conformation of the, CDRH3 loop in each molecule.
About this Structure
1WC7 is a Single protein structure of sequence from Mus musculus with IOD and PP3 as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a pseudomerohedrally twinned monoclinic crystal form of a pyridoxal phosphate-dependent catalytic antibody., Golinelli-Pimpaneau B, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):472-6. Epub 2005, Mar 24. PMID:15805602
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