This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1znm
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1znm.gif|left|200px]]<br /> | + | [[Image:1znm.gif|left|200px]]<br /><applet load="1znm" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1znm" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1znm" /> | caption="1znm" /> | ||
'''A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES'''<br /> | '''A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1ZNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1ZNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=BTD:Beta Turn Dipeptide (Type II' Beta Turn Mimetic)'>BTD</scene> and <scene name='pdbsite=ZNB:Zn Binding Site'>ZNB</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZNM OCA]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 29: | ||
[[Category: zn-btd(7]] | [[Category: zn-btd(7]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:42:23 2007'' |
Revision as of 16:32, 18 December 2007
|
A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES
Overview
We have incorporated a bicyclic beta-turn mimetic (BTD; beta-turn, dipeptide) into a zinc finger, creating a zinc finger with an artificial, beta-turn. The designed peptide chelates zinc and has the same fold as the, unmodified native zinc finger (finger 3 of the human YY1 protein). A, combination of 1H NMR and structure calculations reveals that, in, solution, this zinc finger has a fold similar to the known wild-type, crystal structure and to other zinc fingers containing the consensus, sequence X3-Cys-X4-Cys-X12-His-X3-His-X. The peptide was designed with BTD, between the chelating cysteine residues, with BTD forming a type II', beta-turn linking the two strands of a distorted anti-parallel beta-sheet., The C-terminal portion of the peptide forms a helix with zinc, co-ordinating histidine residues on successive turns of the helix. This, work represents a step towards developing methods by which parts of a, target protein may be replaced by peptide mimetics.
About this Structure
1ZNM is a Single protein structure of sequence from [1] with ZN as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Design, synthesis and structure of a zinc finger with an artificial beta-turn., Viles JH, Patel SU, Mitchell JB, Moody CM, Justice DE, Uppenbrink J, Doyle PM, Harris CJ, Sadler PJ, Thornton JM, J Mol Biol. 1998 Jun 19;279(4):973-86. PMID:9642075
Page seeded by OCA on Tue Dec 18 18:42:23 2007
