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Sandbox 20

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Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
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<applet load="1N2C" size="300" color="white" frame="true" align="right" caption="Nitrogenase"/>
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== Exploring the Structure ==
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Click here to see only <scene name='Sandbox_10/1n2c_single_complex/2'>one complex</scene>.
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<applet load='1eme' size='300' frame='true' align='right' caption='Insert caption here' />
 
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GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The chromophore, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.
 
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References
 
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Ormo, M., Cubitt, A.B., Kallio, K., Gross, L.A., Tsien, R.Y., Remington, S.J. (1996) Crystal structure of the Aequorea victoria green fluorescent protein. Science 273: 1392-1395 PMID: 8703075
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HI this is a test
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<applet load='1ema' size='1000' frame='true' align='right' caption='Insert caption here' />
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<scene name='Sandbox_20/Test01_gfp/1'>Chromophore</scene>
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[[Image:3hfl.png| 600 px| aqq]]
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<ref>PMID:8703075</ref>
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==References==
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<references/>

Current revision

Nitrogenase

Drag the structure with the mouse to rotate

Click here to see only .


HI this is a test

Insert caption here

Drag the structure with the mouse to rotate

aqq

[1]

References

  1. ↑ Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075
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