2gx5
From Proteopedia
(New page: 200px<br /><applet load="2gx5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gx5, resolution 1.74Å" /> '''N-terminal GAF domai...) |
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| - | [[Image:2gx5.gif|left|200px]]<br /><applet load="2gx5" size=" | + | [[Image:2gx5.gif|left|200px]]<br /><applet load="2gx5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gx5, resolution 1.74Å" /> | caption="2gx5, resolution 1.74Å" /> | ||
'''N-terminal GAF domain of transcriptional pleiotropic repressor CodY'''<br /> | '''N-terminal GAF domain of transcriptional pleiotropic repressor CodY'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4, PG4, P6G, PCG, PGE and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2GX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PG4:'>PG4</scene>, <scene name='pdbligand=P6G:'>P6G</scene>, <scene name='pdbligand=PCG:'>PCG</scene>, <scene name='pdbligand=PGE:'>PGE</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GX5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:22:06 2008'' |
Revision as of 09:22, 23 January 2008
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N-terminal GAF domain of transcriptional pleiotropic repressor CodY
Overview
CodY is a global regulator of transcription in gram-positive bacteria. It, represses during growth genes required for adaptation to nutrient, limitation, including virulence genes in some human pathogens. CodY, activity is regulated by GTP and branched chain amino acids, metabolites, whose intracellular concentrations drop as cells enter stationary phase., Although CodY has a highly conserved sequence, it has no significant, similarity to proteins of known structure. Here we report crystal, structures of two fragments of CodY from Bacillus subtilis that clearly, constitute its cofactor and DNA binding domains and reveal that CodY is a, chimera of previously observed folding units. The N-terminal, cofactor-binding fragment adopts a fold reminiscent of the GAF domains, found in cyclic nucleotide phosphodiesterases and adenylate cyclases. It, is a dimer stabilized by an intermolecular six alpha-helical bundle that, buries an extensive apolar surface rich in residues invariant in CodY, orthologues. The branched chain amino acid ligands reside in hydrophobic, pockets of each monomer distal to the dimer-forming surface. The structure, of the C-terminal DNA binding domain belongs to the winged, helix-turn-helix family. The implications of the structure for DNA binding, by CodY and its control by cofactor binding are discussed.
About this Structure
2GX5 is a Single protein structure of sequence from Bacillus subtilis with , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of CodY, a GTP- and isoleucine-responsive regulator of stationary phase and virulence in gram-positive bacteria., Levdikov VM, Blagova E, Joseph P, Sonenshein AL, Wilkinson AJ, J Biol Chem. 2006 Apr 21;281(16):11366-73. Epub 2006 Feb 17. PMID:16488888
Page seeded by OCA on Wed Jan 23 11:22:06 2008
Categories: Bacillus subtilis | Single protein | Blagova, E.V. | Levdikov, V.M. | Wilkinson, A.J. | GOL | P6G | PCG | PG4 | PGE | SO4 | Branched chain amino acid binding | Cody | Dna-binding | Gaf domain | Nucleotide-binding | Repressor | Transcription regulation
