1h1m
From Proteopedia
(New page: 200px<br /> <applet load="1h1m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h1m, resolution 1.90Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H1M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_japonicus Aspergillus japonicus]] with NAG, CU, KMP and MPD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.24 1.13.11.24]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H1M OCA]]. | + | 1H1M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_japonicus Aspergillus japonicus]] with NAG, CU, KMP and MPD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Quercetin_2,3-dioxygenase Quercetin 2,3-dioxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.24 1.13.11.24]]. Structure known Active Site: CUA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H1M OCA]]. |
==Reference== | ==Reference== | ||
Anaerobic enzyme.substrate structures provide insight into the reaction mechanism of the copper-dependent quercetin 2,3-dioxygenase., Steiner RA, Kalk KH, Dijkstra BW, Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16625-30. Epub 2002 Dec 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12486225 12486225] | Anaerobic enzyme.substrate structures provide insight into the reaction mechanism of the copper-dependent quercetin 2,3-dioxygenase., Steiner RA, Kalk KH, Dijkstra BW, Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16625-30. Epub 2002 Dec 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12486225 12486225] | ||
[[Category: Aspergillus japonicus]] | [[Category: Aspergillus japonicus]] | ||
| + | [[Category: Quercetin 2,3-dioxygenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dijkstra, B.W.]] | [[Category: Dijkstra, B.W.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:08:00 2007'' |
Revision as of 09:03, 30 October 2007
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CRYSTAL STRUCTURE OF QUERCETIN 2,3-DIOXYGENASE ANAEROBICALLY COMPLEXED WITH THE SUBSTRATE KAEMPFEROL
Overview
Quercetin 2,3-dioxygenase (2,3QD) is the only firmly established copper, dioxygenase known so far. Depending solely on a mononuclear Cu center, it, catalyzes the breakage of the O-heterocycle of flavonols, producing more, easily degradable phenolic carboxylic acid ester derivatives. In the, enzymatic process, two CC bonds are broken and concomitantly carbon, monoxide is released. The x-ray structures of Aspergillus japonicus 2,3QD, anaerobically complexed with the substrate kaempferol and the natural, substrate quercetin have been determined at 1.90- and 1.75-A resolution, respectively. Flavonols coordinate to the copper ion as monodentate, ligands through their 3OH group. They occupy a shallow and overall, hydrophobic cavity proximal to the metal center. As a result of a van der, Waals ... [(full description)]
About this Structure
1H1M is a [Single protein] structure of sequence from [Aspergillus japonicus] with NAG, CU, KMP and MPD as [ligands]. Active as [Quercetin 2,3-dioxygenase], with EC number [1.13.11.24]. Structure known Active Site: CUA. Full crystallographic information is available from [OCA].
Reference
Anaerobic enzyme.substrate structures provide insight into the reaction mechanism of the copper-dependent quercetin 2,3-dioxygenase., Steiner RA, Kalk KH, Dijkstra BW, Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16625-30. Epub 2002 Dec 16. PMID:12486225
Page seeded by OCA on Tue Oct 30 11:08:00 2007
Categories: Aspergillus japonicus | Quercetin 2,3-dioxygenase | Single protein | Dijkstra, B.W. | Steiner, R.A. | CU | KMP | MPD | NAG | Copper | Dioxygenase | Flavonol | Oxidoreductase
