2bfn
From Proteopedia
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| - | [[Image:2bfn.gif|left|200px]]<br /><applet load="2bfn" size=" | + | [[Image:2bfn.gif|left|200px]]<br /><applet load="2bfn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bfn, resolution 1.600Å" /> | caption="2bfn, resolution 1.600Å" /> | ||
'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''<br /> | '''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''<br /> | ||
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| + | ==Overview== | ||
| + | 1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound., Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage, of a carbon-halogen bond in a wide range of organic halogenated compounds., Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a, long time, been considered inactive with TCP, since the reaction cannot be, easily detected by conventional analytical methods. Here we demonstrate, detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP, using X-ray crystallography and microcalorimetry. This observation makes, LinB a useful starting material for the development of a new biocatalyst, toward TCP by protein engineering. Microcalorimetry is proposed to be a, universal method for the detection of weak enzymatic activities. Detection, of these activities is becoming increasingly important for engineering, novel biocatalysts using the scaffolds of proteins with promiscuous, activities. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2BFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=D2P:'>D2P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:D2p Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Cl Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Ca Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Ca Binding Site For Chain A'>AC4</scene> and <scene name='pdbsite=AC5:Ca Binding Site For Chain A'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFN OCA]. |
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| + | ==Reference== | ||
| + | Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17259360 17259360] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Sphingomonas paucimobilis]] | ||
[[Category: Banas, P.]] | [[Category: Banas, P.]] | ||
[[Category: Bohac, M.]] | [[Category: Bohac, M.]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:30:44 2008'' |
Revision as of 09:30, 23 January 2008
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THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.
Overview
1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound., Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage, of a carbon-halogen bond in a wide range of organic halogenated compounds., Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a, long time, been considered inactive with TCP, since the reaction cannot be, easily detected by conventional analytical methods. Here we demonstrate, detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP, using X-ray crystallography and microcalorimetry. This observation makes, LinB a useful starting material for the development of a new biocatalyst, toward TCP by protein engineering. Microcalorimetry is proposed to be a, universal method for the detection of weak enzymatic activities. Detection, of these activities is becoming increasingly important for engineering, novel biocatalysts using the scaffolds of proteins with promiscuous, activities.
About this Structure
2BFN is a Single protein structure of sequence from [1] with , and as ligands. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.
Reference
Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:17259360
Page seeded by OCA on Wed Jan 23 11:30:44 2008
Categories: Single protein | Banas, P. | Bohac, M. | Damborsky, J. | Jerabek, P. | Otyepka, M. | Vevodova, J. | CA | CL | D2P | 1 | 2 | 3-trichloropropane | Alpha/beta-hydrolase | Haloalkane dehalogenase linb | Hydrolase
