2jer
From Proteopedia
(New page: 200px<br /> <applet load="2jer" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jer, resolution 1.65Å" /> '''AGMATINE DEIMINASE ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2JER is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]] with AGT and AGT as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 2J2T. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.12 3.5.3.12]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JER OCA]]. | + | 2JER is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]] with AGT and AGT as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 2J2T. Active as [[http://en.wikipedia.org/wiki/Agmatine_deiminase Agmatine deiminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.12 3.5.3.12]]. Structure known Active Site: CATALYTIC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JER OCA]]. |
==Reference== | ==Reference== | ||
The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction., Llacer JL, Polo LM, Tavarez S, Alarcon B, Hilario R, Rubio V, J Bacteriol. 2007 Feb;189(4):1254-65. Epub 2006 Oct 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17028272 17028272] | The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction., Llacer JL, Polo LM, Tavarez S, Alarcon B, Hilario R, Rubio V, J Bacteriol. 2007 Feb;189(4):1254-65. Epub 2006 Oct 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17028272 17028272] | ||
| + | [[Category: Agmatine deiminase]] | ||
[[Category: Enterococcus faecalis]] | [[Category: Enterococcus faecalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tetramer]] | [[Category: tetramer]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:09:59 2007'' |
Revision as of 09:05, 30 October 2007
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AGMATINE DEIMINASE OF ENTEROCOCCUS FAECALIS CATALYZING ITS REACTION.
Overview
Enterococcus faecalis makes ATP from agmatine in three steps catalyzed by, agmatine deiminase (AgDI), putrescine transcarbamylase (PTC), and, carbamate kinase (CK). An antiporter exchanges putrescine for agmatine. We, have cloned the E. faecalis ef0732 and ef0734 genes of the reported gene, cluster for agmatine catabolism, overexpressed them in Escherichia coli, purified the products, characterized them functionally as PTC and AgDI, and crystallized and X-ray diffracted them. The 1.65-Angstroms-resolution, structure of AgDI forming a covalent adduct with an agmatine-derived, amidine reactional intermediate is described. We provide definitive, identification of the gene cluster for agmatine catabolism and confirm, that ornithine is a genuine but poor PTC substrate, suggesting that PTC, ... [(full description)]
About this Structure
2JER is a [Single protein] structure of sequence from [Enterococcus faecalis] with AGT and AGT as [ligands]. This structure superseeds the now removed PDB entry 2J2T. Active as [Agmatine deiminase], with EC number [3.5.3.12]. Structure known Active Site: CATALYTIC. Full crystallographic information is available from [OCA].
Reference
The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction., Llacer JL, Polo LM, Tavarez S, Alarcon B, Hilario R, Rubio V, J Bacteriol. 2007 Feb;189(4):1254-65. Epub 2006 Oct 6. PMID:17028272
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