1a8u
From Proteopedia
(New page: 200px<br /> <applet load="1a8u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8u, resolution 1.60Å" /> '''CHLOROPEROXIDASE T/...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1A8U is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]] with SO4 and BEZ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8U OCA]]. | + | 1A8U is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]] with SO4 and BEZ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10]]. Structure known Active Sites: NUA and NUB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8U OCA]]. |
==Reference== | ==Reference== | ||
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9642069 9642069] | Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9642069 9642069] | ||
| + | [[Category: Chloride peroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces aureofaciens]] | [[Category: Streptomyces aureofaciens]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:12:33 2007'' |
Revision as of 09:07, 30 October 2007
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CHLOROPEROXIDASE T/BENZOATE COMPLEX
Overview
The structures of cofactor-free haloperoxidases from Streptomyces, aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been, determined at resolutions between 1.9 A and 1.5 A. The structures of two, enzymes complexed with benzoate or propionate identify the binding site, for the organic acids which are required for the haloperoxidase activity., Based on these complexes and on the structure of an inactive variant, a, reaction mechanism is proposed for the halogenation reaction with, peroxoacid and hypohalous acid as reaction intermediates. Comparison of, the structures suggests that a specific halide binding site is absent in, the enzymes but that hydrophobic organic compounds may fit into the active, site pocket for halogenation at preferential sites.
About this Structure
1A8U is a [Single protein] structure of sequence from [Streptomyces aureofaciens] with SO4 and BEZ as [ligands]. Active as [Chloride peroxidase], with EC number [1.11.1.10]. Structure known Active Sites: NUA and NUB. Full crystallographic information is available from [OCA].
Reference
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069
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