1mdl
From Proteopedia
(New page: 200px<br /> <applet load="1mdl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mdl, resolution 1.85Å" /> '''MANDELATE RACEMASE ...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MDL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG, RMN and SMN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MDL OCA]]. | + | 1MDL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG, RMN and SMN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Mandelate_racemase Mandelate racemase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MDL OCA]]. |
==Reference== | ==Reference== | ||
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the K166R mutant., Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1995 Mar 7;34(9):2788-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893690 7893690] | Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the K166R mutant., Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1995 Mar 7;34(9):2788-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893690 7893690] | ||
| + | [[Category: Mandelate racemase]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 24: | ||
[[Category: mandelate pathway]] | [[Category: mandelate pathway]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:18:28 2007'' |
Revision as of 09:13, 30 October 2007
|
MANDELATE RACEMASE MUTANT K166R CO-CRYSTALLIZED WITH (R)-MANDELATE
Overview
On the basis of the available high-resolution structures of mandelate, racemase (MR) from Pseudomonas putida [Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., & Petsko, G. A. (1994) Biochemistry 33, 635-643], Lys 166 and, His 297 are positioned appropriately to participate in catalysis as, acid/base catalysts that either abstract the alpha-proton from the, enantiomers of mandelate to form an enolic intermediate or protonate the, enolic intermediate to form the enantiomers of mandelate, with Lys 166, participating as the (S)-specific acid/base catalyst and His 297, participating as the (R)-specific acid/base catalyst. In this paper we, report the structural and mechanistic properties of the mutant in which, Lys 166 has ... [(full description)]
About this Structure
1MDL is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with MG, RMN and SMN as [ligands]. Active as [Mandelate racemase], with EC number [5.1.2.2]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [OCA].
Reference
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the K166R mutant., Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1995 Mar 7;34(9):2788-97. PMID:7893690
Page seeded by OCA on Tue Oct 30 11:18:28 2007
