This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1mra
From Proteopedia
(New page: 200px<br /> <applet load="1mra" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mra, resolution 2.1Å" /> '''MANDELATE RACEMASE M...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MRA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MRA OCA]]. | + | 1MRA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Mandelate_racemase Mandelate racemase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MRA OCA]]. |
==Reference== | ==Reference== | ||
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8639525 8639525] | Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8639525 8639525] | ||
| + | [[Category: Mandelate racemase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 24: | ||
[[Category: mandelate pathway]] | [[Category: mandelate pathway]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:19:22 2007'' |
Revision as of 09:14, 30 October 2007
|
MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE
Overview
On the basis of the available high-resolution structures of mandelate, racemase (MR) from Pseudomonas putida [Landro, J.A., Gerlt, J.A., Kozarich, J.W., Koo, C.W., Shah, V.J., Kenyon, G.L., Neidhart, D.J., Fujita, J., & Petsko, G.A. (1994) Biochemistry 33, 635-643], Lys 166 and, His 297 are positioned appropriately to participate in catalysis as, acid/base catalysts, with Lys 166 participating as the (S)-specific, acid/base catalyst and His 297 participating as the (R)-specific acid/base, catalyst. The dependence of kcat on pH for the racemization of both (R)-, and (S)-mandelates suggests that the pKaS of the conjugate acids of Lys, 166 and His 297 are both approximately 6.4 [Landro, J.A., Kallarakal, A.T., Ransom, S.C., Gerlt, J.A., Kozarich, J.W., Neidhart, D.J., Kenyon, G.L. (1991) ... [(full description)]
About this Structure
1MRA is a [Single protein] structure of sequence from [Pseudomonas putida] with MG and APG as [ligands]. Active as [Mandelate racemase], with EC number [5.1.2.2]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [OCA].
Reference
Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant., Schafer SL, Barrett WC, Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1996 May 7;35(18):5662-9. PMID:8639525
Page seeded by OCA on Tue Oct 30 11:19:22 2007
