This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2pns
From Proteopedia
(New page: 200px<br /><applet load="2pns" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pns, resolution 1.90Å" /> '''1.9 Angstrom resolut...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2pns.jpg|left|200px]]<br /><applet load="2pns" size=" | + | [[Image:2pns.jpg|left|200px]]<br /><applet load="2pns" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2pns, resolution 1.90Å" /> | caption="2pns, resolution 1.90Å" /> | ||
'''1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence'''<br /> | '''1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PNS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tabernaemontana_divaricata Tabernaemontana divaricata] with PO4 and THJ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2PNS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tabernaemontana_divaricata Tabernaemontana divaricata] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=THJ:'>THJ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNS OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: thermostable]] | [[Category: thermostable]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:50:26 2008'' |
Revision as of 10:50, 23 January 2008
|
1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence
Overview
We report here the cloning and characterization of the entire cDNA of a, papain-like cysteine protease from a tropical flowering plant. The 1098-bp, ORF of the cDNA codify a protease precursor having a signal peptide of 19, amino acids, a cathepsin-L like N-terminal proregion of 114 amino acids, a, mature enzyme part of 208 amino acids and a C-terminal proregion of 24, amino acids. The derived amino acid sequence of the mature part tallies, with the thermostable cysteine protease Ervatamin-C-as was aimed at. The, C-terminal proregion of the protease has altogether a different sequence, pattern not observed in other members of the family and it contains a, negatively charged helical zone. The three-dimensional model of the, precursor, based on the homology modeling and X-ray structure, shows that, the extended peptide stretch region of the N-terminal propeptide, covering, the interdomain cleft, contains protruding side chains of positively, charged residues. This study also indicates that the negatively charged, zone of C-terminal propeptide may interact with the positively charged, zone of the N-terminal propeptide in a cooperative manner in the, maturation process of this enzyme.
About this Structure
2PNS is a Protein complex structure of sequences from Tabernaemontana divaricata with and as ligands. Full crystallographic information is available from OCA.
Reference
A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies., Ghosh R, Dattagupta JK, Biswas S, Biochem Biophys Res Commun. 2007 Nov 3;362(4):965-70. Epub 2007 Aug 28. PMID:17767923
Page seeded by OCA on Wed Jan 23 12:50:26 2008
