1h6m
From Proteopedia
(New page: 200px<br /> <applet load="1h6m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h6m, resolution 1.64Å" /> '''COVALENT GLYCOSYL-E...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H6M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]] with NA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H6M OCA]]. | + | 1H6M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]] with NA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Lysozyme Lysozyme]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Structure known Active Sites: NA, NAG and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H6M OCA]]. |
==Reference== | ==Reference== | ||
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11518970 11518970] | Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11518970 11518970] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
| + | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davies, G.J.]] | [[Category: Davies, G.J.]] | ||
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[[Category: mechanism]] | [[Category: mechanism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:32:18 2007'' |
Revision as of 09:27, 30 October 2007
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COVALENT GLYCOSYL-ENZYME INTERMEDIATE OF HEN EGG WHITE LYSOZYME
Overview
Hen egg-white lysozyme (HEWL) was the first enzyme to have its, three-dimensional structure determined by X-ray diffraction techniques. A, catalytic mechanism, featuring a long-lived oxocarbenium-ion intermediate, was proposed on the basis of model-building studies. The 'Phillips', mechanism is widely held as the paradigm for the catalytic mechanism of, beta-glycosidases that cleave glycosidic linkages with net retention of, configuration of the anomeric centre. Studies with other retaining, beta-glycosidases, however, provide strong evidence pointing to a common, mechanism for these enzymes that involves a covalent glycosyl-enzyme, intermediate, as previously postulated. Here we show, in three different, cases using electrospray ionization mass spectrometry, a catalytically, competent ... [(full description)]
About this Structure
1H6M is a [Single protein] structure of sequence from [Gallus gallus] with NA as [ligand]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Sites: NA, NAG and NUC. Full crystallographic information is available from [OCA].
Reference
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:11518970
Page seeded by OCA on Tue Oct 30 11:32:18 2007
