This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1h6m
From Proteopedia
(New page: 200px<br /> <applet load="1h6m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h6m, resolution 1.64Å" /> '''COVALENT GLYCOSYL-E...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1H6M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]] with NA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H6M OCA]]. | + | 1H6M is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]] with NA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Lysozyme Lysozyme]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Structure known Active Sites: NA, NAG and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H6M OCA]]. |
==Reference== | ==Reference== | ||
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11518970 11518970] | Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11518970 11518970] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
| + | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davies, G.J.]] | [[Category: Davies, G.J.]] | ||
| Line 24: | Line 25: | ||
[[Category: mechanism]] | [[Category: mechanism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:32:18 2007'' |
Revision as of 09:27, 30 October 2007
|
COVALENT GLYCOSYL-ENZYME INTERMEDIATE OF HEN EGG WHITE LYSOZYME
Overview
Hen egg-white lysozyme (HEWL) was the first enzyme to have its, three-dimensional structure determined by X-ray diffraction techniques. A, catalytic mechanism, featuring a long-lived oxocarbenium-ion intermediate, was proposed on the basis of model-building studies. The 'Phillips', mechanism is widely held as the paradigm for the catalytic mechanism of, beta-glycosidases that cleave glycosidic linkages with net retention of, configuration of the anomeric centre. Studies with other retaining, beta-glycosidases, however, provide strong evidence pointing to a common, mechanism for these enzymes that involves a covalent glycosyl-enzyme, intermediate, as previously postulated. Here we show, in three different, cases using electrospray ionization mass spectrometry, a catalytically, competent ... [(full description)]
About this Structure
1H6M is a [Single protein] structure of sequence from [Gallus gallus] with NA as [ligand]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Sites: NA, NAG and NUC. Full crystallographic information is available from [OCA].
Reference
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:11518970
Page seeded by OCA on Tue Oct 30 11:32:18 2007
