1muc
From Proteopedia
(New page: 200px<br /> <applet load="1muc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1muc, resolution 1.85Å" /> '''STRUCTURE OF MUCONA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1MUC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1MLE. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.1 5.5.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUC OCA]]. | + | 1MUC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1MLE. Active as [[http://en.wikipedia.org/wiki/Muconate_cycloisomerase Muconate cycloisomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.1 5.5.1.1]]. Structure known Active Sites: MNA and MNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUC OCA]]. |
==Reference== | ==Reference== | ||
The refined X-ray structure of muconate lactonizing enzyme from Pseudomonas putida PRS2000 at 1.85 A resolution., Helin S, Kahn PC, Guha BL, Mallows DG, Goldman A, J Mol Biol. 1995 Dec 15;254(5):918-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7500361 7500361] | The refined X-ray structure of muconate lactonizing enzyme from Pseudomonas putida PRS2000 at 1.85 A resolution., Helin S, Kahn PC, Guha BL, Mallows DG, Goldman A, J Mol Biol. 1995 Dec 15;254(5):918-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7500361 7500361] | ||
| + | [[Category: Muconate cycloisomerase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: muconate lactonizing enzyme]] | [[Category: muconate lactonizing enzyme]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:34:40 2007'' |
Revision as of 09:29, 30 October 2007
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STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION
Overview
We report here the refined X-ray crystal structure of muconate lactonizing, enzyme (MLE) from Pseudomonas putida PRS2000 at a resolution of 1.85 A, with an R-factor of 16.8%. An enzyme from the beta-ketoadipate pathway, MLE catalyses the conversion of cis,cis-muconate to muconolactone. It is a, homo-octamer, one monomer consisting of 373 amino acid residues. MLE has, two large domains and a C-terminal subdomain: an alpha + beta domain, an, alpha beta-barrel domain and a C-terminal meandering subdomain. The alpha, beta-barrel domain is highly irregular. Its structure is (beta/alpha)7, beta, with the structural role of the last alpha-helix being replaced by, both the C-terminal subdomain and part of the N-terminal domain. The, fifth, seventh and eighth barrel strands are unusual because ... [(full description)]
About this Structure
1MUC is a [Single protein] structure of sequence from [Pseudomonas putida] with MN as [ligand]. This structure superseeds the now removed PDB entry 1MLE. Active as [Muconate cycloisomerase], with EC number [5.5.1.1]. Structure known Active Sites: MNA and MNB. Full crystallographic information is available from [OCA].
Reference
The refined X-ray structure of muconate lactonizing enzyme from Pseudomonas putida PRS2000 at 1.85 A resolution., Helin S, Kahn PC, Guha BL, Mallows DG, Goldman A, J Mol Biol. 1995 Dec 15;254(5):918-41. PMID:7500361
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