2jfe
From Proteopedia
(New page: 200px<br /> <applet load="2jfe" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jfe, resolution 2.70Å" /> '''THE CRYSTAL STRUCTU...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2JFE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JFE OCA]]. | + | 2JFE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JFE OCA]]. |
==Reference== | ==Reference== | ||
The Crystal Structure of Human Cytosolic beta-Glucosidase Unravels the Substrate Aglycone Specificity of a Family 1 Glycoside Hydrolase., Tribolo S, Berrin JG, Kroon PA, Czjzek M, Juge N, J Mol Biol. 2007 Jul 27;370(5):964-75. Epub 2007 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17555766 17555766] | The Crystal Structure of Human Cytosolic beta-Glucosidase Unravels the Substrate Aglycone Specificity of a Family 1 Glycoside Hydrolase., Tribolo S, Berrin JG, Kroon PA, Czjzek M, Juge N, J Mol Biol. 2007 Jul 27;370(5):964-75. Epub 2007 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17555766 17555766] | ||
| + | [[Category: Beta-glucosidase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: polymorphism]] | [[Category: polymorphism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:41:07 2007'' |
Revision as of 09:36, 30 October 2007
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THE CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC BETA-GLUCOSIDASE
Overview
Human cytosolic beta-glucosidase (hCBG) is a xenobiotic-metabolizing, enzyme that hydrolyses certain flavonoid glucosides, with specificity, depending on the aglycone moiety, the type of sugar and the linkage, between them. In this study, the substrate preference of this enzyme was, investigated by mutational analysis, X-ray crystallography and homology, modelling. The crystal structure of hCBG was solved by the molecular, replacement method and refined at 2.7 A resolution. The main-chain fold of, the enzyme belongs to the (beta/alpha)(8) barrel structure, which is, common to family 1 glycoside hydrolases. The active site is located at the, bottom of a pocket (about 16 A deep) formed by large surface loops, surrounding the C termini of the barrel of beta-strands. As for all the, clan of ... [(full description)]
About this Structure
2JFE is a [Single protein] structure of sequence from [Homo sapiens]. Active as [Beta-glucosidase], with EC number [3.2.1.21]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The Crystal Structure of Human Cytosolic beta-Glucosidase Unravels the Substrate Aglycone Specificity of a Family 1 Glycoside Hydrolase., Tribolo S, Berrin JG, Kroon PA, Czjzek M, Juge N, J Mol Biol. 2007 Jul 27;370(5):964-75. Epub 2007 May 18. PMID:17555766
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