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=Structure and Function=
=Structure and Function=
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The crystal structure of sphingomyelinase has been solved using ''Listeria ivanovii'' and ''Bacillus cereus (Bc-SMase)''<ref name="gp4">PMID: 16595670 </ref>.
=Mechanism=
=Mechanism=
=Importance of SMase=
=Importance of SMase=
=References=
=References=
<references/>
<references/>

Revision as of 16:31, 23 March 2011

This Sandbox is Reserved from January 10, 2010, through April 10, 2011 for use in BCMB 307-Proteins course taught by Andrea Gorrell at the University of Northern British Columbia, Prince George, BC, Canada.
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PDB ID 2dds

Drag the structure with the mouse to rotate
2dds, resolution 1.80Å ()
Ligands:
Activity: Sphingomyelin phosphodiesterase, with EC number 3.1.4.12
Related: 2ddr, 2ddt
Resources: FirstGlance, OCA, PDBsum, RCSB, TOPSAN
Coordinates: save as pdb, mmCIF, xml


Contents

Introduction

Sphingomyelin phosphodiesterase (Sphingomyelinase): (SMase) is an enzyme which catalyzes the hydrolysis of sphingomyelin to ceramide and phosphocholine [1]. This enzyme has become the object of renewed interest since the discovery of the sphingomyelin signal transduction pathway which is involved in apoptosis. This pathway is initiated by a neutral sphingomyelinase hydrolysis of sphingomyelin in the plasma membrane to generate ceramide. Ceramide acts as a secondary messenger which causes the stimulation of the cascade effect of kinases and transcription factors which activate programmed cell death[2]. There are 6 known types of sphingomyelinases[3]. :

Acid sphingomyelinase (aSMase) -

Secretory sphingomyelinase (sSMase) -

Neutral Mg2+-dependent sphingomyelinases (nSMase) -

Neutral Mg2+-independent sphingomyelinases -

Alkaline sphingomyelinase -

Bacterial sphingomyelinase -

Structure and Function

The crystal structure of sphingomyelinase has been solved using Listeria ivanovii and Bacillus cereus (Bc-SMase)[4].

Mechanism

Importance of SMase

References

  1. ↑ Ago H, Oda M, Takahashi M, Tsuge H, Ochi S, Katunuma N, Miyano M, Sakurai J. Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from Bacillus cereus. J Biol Chem. 2006 Jun 9;281(23):16157-67. Epub 2006 Apr 4. PMID:16595670 doi:10.1074/jbc.M601089200
  2. ↑ Kolesnick RN, Haimovitz-Friedman A, Fuks Z. The sphingomyelin signal transduction pathway mediates apoptosis for tumor necrosis factor, Fas, and ionizing radiation. Biochem Cell Biol. 1994 Nov-Dec;72(11-12):471-4. PMID:7544586
  3. ↑ Goni FM, Alonso A. Sphingomyelinases: enzymology and membrane activity. FEBS Lett. 2002 Oct 30;531(1):38-46. PMID:12401200
  4. ↑ Ago H, Oda M, Takahashi M, Tsuge H, Ochi S, Katunuma N, Miyano M, Sakurai J. Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from Bacillus cereus. J Biol Chem. 2006 Jun 9;281(23):16157-67. Epub 2006 Apr 4. PMID:16595670 doi:10.1074/jbc.M601089200
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