Colicin K
From Proteopedia
(New page: Colicin K is a type of Colicin, a bacteriocin made by "E. Coli" which acts against other nearby "E. Coli" to kill them by forming a pore in the membrane, leading to depolarisation of t...) |
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| - | Colicin K is a type of [[Colicin]], a bacteriocin made by | + | Colicin K is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them by forming a pore in the membrane, leading to depolarisation of the membrane which kills the cell. |
==Synthesis and release== | ==Synthesis and release== | ||
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| + | The colicin K operon is encoded on a plasmid in the ''E. coli'' cell. The operon encodes the colicin itself, its [[Colicin Immunity Protein]] and a protein for release of the colicin into the surrounding area. The immunity protein acts by inserting into the membrane of the colicin-producing cell, and prevents the colicin from forming a pore in the membrane. | ||
==Mechanism of uptake== | ==Mechanism of uptake== | ||
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| + | The receptor binding domain of Colicin K binds to the outer membrane protein Tsx of the target cell, a constitutively expressed protein parasitised by the colicin. The translocation domain then recruits proteins from the [[Tol]] family to translocate across the membrane and into the cytoplasm, through a mechanism as yet unidentified. | ||
==Killing Activities== | ==Killing Activities== | ||
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| + | The cytotoxic domain forms a pore in the membrane of the target cell, causing it to depolarise. This prevents the cell from being able to synthesise ATP, ultimately leading to the death of the cell. | ||
Current revision
Colicin K is a type of Colicin, a bacteriocin made by E. coli which acts against other nearby E. coli to kill them by forming a pore in the membrane, leading to depolarisation of the membrane which kills the cell.
Synthesis and release
The colicin K operon is encoded on a plasmid in the E. coli cell. The operon encodes the colicin itself, its Colicin Immunity Protein and a protein for release of the colicin into the surrounding area. The immunity protein acts by inserting into the membrane of the colicin-producing cell, and prevents the colicin from forming a pore in the membrane.
Mechanism of uptake
The receptor binding domain of Colicin K binds to the outer membrane protein Tsx of the target cell, a constitutively expressed protein parasitised by the colicin. The translocation domain then recruits proteins from the Tol family to translocate across the membrane and into the cytoplasm, through a mechanism as yet unidentified.
Killing Activities
The cytotoxic domain forms a pore in the membrane of the target cell, causing it to depolarise. This prevents the cell from being able to synthesise ATP, ultimately leading to the death of the cell.
