1h0j

From Proteopedia

Revision as of 09:49, 30 October 2007

STRUCTURAL BASIS OF THE MEMBRANE-INDUCED CARDIOTOXIN A3 OLIGOMERIZATION

Overview

Cobra cardiotoxins (CTXs) have previously been shown to induce membrane, fusion of vesicles formed by phospholipids such as cardiolipin or, sphingomyelin. CTX can also form a pore in membrane bilayers containing a, anionic lipid such as phosphatidylserine or phosphatidylglycerol. Herein, we show that the interaction of CTX with negatively charged lipids causes, CTX dimerization, an important intermediate for the eventual, oligomerization of CTX during the CTX-induced fusion and pore formation, process. The structural basis of the lipid-induced oligomerization of CTX, A3, a major CTX from Naja atra, is then illustrated by the crystal, structure of CTX A3 in complex with SDS; SDS likely mimics anionic lipids, of the membrane under micelle conditions at 1.9-A resolution. The crystal, packing ... [(full description)]

About this Structure

1H0J is a [Single protein] structure of sequence from [Naja atra] with SDS as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural basis of membrane-induced cardiotoxin A3 oligomerization., Forouhar F, Huang WN, Liu JH, Chien KY, Wu WG, Hsiao CD, J Biol Chem. 2003 Jun 13;278(24):21980-8. Epub 2003 Mar 26. PMID:12660250

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