Aldehyde dehydrogenase

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Line 20: Line 20:
[[1ag8]] – bALDH NAD binding domain - bovine
[[1ag8]] – bALDH NAD binding domain - bovine
-
Binary complex
+
''Binary complex''
[[3inj]], [[3inl]] - hALDH2 residues 18-517 + agonist<BR />
[[3inj]], [[3inl]] - hALDH2 residues 18-517 + agonist<BR />
Line 28: Line 28:
[[1a4z]] – bALDH2 NAD-binding domain + NAD<BR />
[[1a4z]] – bALDH2 NAD-binding domain + NAD<BR />
-
Ternary complex
+
''Ternary complex''
[[1o01]] - hALDH2 residues 18-517 + NADH + aldehyde
[[1o01]] - hALDH2 residues 18-517 + NADH + aldehyde
Line 40: Line 40:
[[2w8n]], [[2w8o]], [[2w8r]] – Succinate–semi-hALDH family 5<BR />
[[2w8n]], [[2w8o]], [[2w8r]] – Succinate–semi-hALDH family 5<BR />
[[2w8p]] - Succinate–semi-hALDH family 5 (mutant) <BR />
[[2w8p]] - Succinate–semi-hALDH family 5 (mutant) <BR />
-
[[3r31]] – Betaine-ALDH – Agrobacterium tumefaciens<BR />
+
[[3r31]] – Betaine-ALDH – ''Agrobacterium tumefaciens''<BR />
-
[[3ed6]] - Betaine-SaALDH – Staphylococcus aureus<BR />
+
[[3ed6]] - Betaine-SaALDH – ''Staphylococcus aureus''<BR />
[[1a4s]] - Betaine-cALDH - cod<BR />
[[1a4s]] - Betaine-cALDH - cod<BR />
[[3llg]] – Aspartate-semi-MtALDH<BR />
[[3llg]] – Aspartate-semi-MtALDH<BR />
[[2cq8]] - Formyltetrahydrofolate-hALDH PP-binding domain - NMR<BR />
[[2cq8]] - Formyltetrahydrofolate-hALDH PP-binding domain - NMR<BR />
[[2nac]] – Formate-PsALDH<BR />
[[2nac]] – Formate-PsALDH<BR />
-
[[3i44]] – ALDH – Bartonella henselae<BR />
+
[[3i44]] – ALDH – ''Bartonella henselae''<BR />
-
[[3ek1]] – ALDH – Brucella melitensis
+
[[3ek1]] – ALDH – ''Brucella melitensis''
-
Binary complexes
+
''Binary complexes''
[[2w8q]] - Succinate–semi-hALDH family 5 + succinic acid<BR />
[[2w8q]] - Succinate–semi-hALDH family 5 + succinic acid<BR />
Line 56: Line 56:
[[2j6l]] – Aminoadipate-semi-hALDH family 7 + NAD<BR />
[[2j6l]] – Aminoadipate-semi-hALDH family 7 + NAD<BR />
[[2cfi]] - Formyltetrahydrofolate-hALDH hydrolase domain + formyltetrahydropterin<BR />
[[2cfi]] - Formyltetrahydrofolate-hALDH hydrolase domain + formyltetrahydropterin<BR />
-
[[2nad]] - Formate-PsALDH + NAD – Pseudomonas<BR />
+
[[2nad]] - Formate-PsALDH + NAD – ''Pseudomonas''<BR />
[[1bi9]] – Retinal-rALDH2 + NAD<BR />
[[1bi9]] – Retinal-rALDH2 + NAD<BR />
-
[[3l4p]], [[1sij]] – ALDH + AsO3 – Desulfovibrio gigas
+
[[3l4p]], [[1sij]] – ALDH + AsO3 – ''Desulfovibrio gigas''
-
Ternary complexes
+
''Ternary complexes''
-
[[2gsd]] – Formate-ALDH + NAD + azide – Moraxella<BR />
+
[[2gsd]] – Formate-ALDH + NAD + azide – ''Moraxella''<BR />
[[1nvm]] – Acetaldehyde-PsALDH + 4-hydroxy-oxovalerate aldolase + NAD<BR />
[[1nvm]] – Acetaldehyde-PsALDH + 4-hydroxy-oxovalerate aldolase + NAD<BR />
-
[[1llu]] - PaALDH + NAD + alcohol – Pseudomonas aeruginosa
+
[[1llu]] - PaALDH + NAD + alcohol – ''Pseudomonas aeruginosa''
===NAD-dependent glyceraldehydes-3-phosphate dehydrogenase===
===NAD-dependent glyceraldehydes-3-phosphate dehydrogenase===
-
[[1vsu]] – CpGAPDH – Cryptosporidium parvum<BR />
+
[[1vsu]] – CpGAPDH – ''Cryptosporidium parvum''<BR />
-
[[1ywg]] – PfGAPDH – Plasmodium falciparum<BR />
+
[[1ywg]] – PfGAPDH – ''Plasmodium falciparum''<BR />
-
[[3cmc]] - GAPDH – Bacillus stearothermophilus<BR />
+
[[3cmc]] - GAPDH – ''Bacillus stearothermophilus''<BR />
-
[[2ep7]] – GAPDH – Aquifex aeolicus<BR />
+
[[2ep7]] – GAPDH – ''Aquifex aeolicus''<BR />
-
[[2i5p]] – GAPDH – Kluyveromyces marxianus<BR />
+
[[2i5p]] – GAPDH – ''Kluyveromyces marxianus''<BR />
-
[[1obf]] – GAPDH – Achromobacter xylosoxidans<BR />
+
[[1obf]] – GAPDH – ''Achromobacter xylosoxidans''<BR />
-
[[1crw]] – PvGAPDH – Palinurus versicolor<BR />
+
[[1crw]] – PvGAPDH – ''Palinurus versicolor''<BR />
-
[[1b7g]] – GAPDH – Sulfolobus solfataricus<BR />
+
[[1b7g]] – GAPDH – ''Sulfolobus solfataricus''<BR />
-
[[2gd1]] – GsGAPDH – Geobacillus stearothermophilus<BR />
+
[[2gd1]] – GsGAPDH – ''Geobacillus stearothermophilus''<BR />
-
[[3e6a]] – OsGAPDH – Oryza sativa<BR />
+
[[3e6a]] – OsGAPDH – ''Oryza sativa''<BR />
[[4gpd]], [[1gpd]] – GAPDH – lobster<BR />
[[4gpd]], [[1gpd]] – GAPDH – lobster<BR />
[[3l6o]], [[3lc7]] – SaGAPDH<BR />
[[3l6o]], [[3lc7]] – SaGAPDH<BR />
-
[[1dc3]], [[1dc5]], [[1s7c]] – EcGAPDH - Escherichia coli
+
[[1dc3]], [[1dc5]], [[1s7c]] – EcGAPDH - ''Escherichia coli''
''GAPDH binary complex''
''GAPDH binary complex''
[[1k3t]] - TcGAPDH + inhibitor<BR />
[[1k3t]] - TcGAPDH + inhibitor<BR />
-
[[3hja]] - GAPDH + NAD – Borellia burgdorferi<BR />
+
[[3hja]] - GAPDH + NAD – ''Borellia burgdorferi''<BR />
[[1vsv]], [[3cps]] - CpGAPDH + NAD<BR />
[[1vsv]], [[3cps]] - CpGAPDH + NAD<BR />
[[2vyn]], [[2vyv]] - rGAPDH residues 102-432 + EcGAPDH + NAD<BR />
[[2vyn]], [[2vyv]] - rGAPDH residues 102-432 + EcGAPDH + NAD<BR />
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[[1dss]], [[1szj]] - PvGAPDH NAD-binding and catalytic domains+ NAD<BR />
[[1dss]], [[1szj]] - PvGAPDH NAD-binding and catalytic domains+ NAD<BR />
[[1ihy]] – PvGAPDH + ADP-ribose<BR />
[[1ihy]] – PvGAPDH + ADP-ribose<BR />
-
[[1gyq]], [[1gyp]] - LmGAPDH + NAD derivative – Leishmania mexicana<BR />
+
[[1gyq]], [[1gyp]] - LmGAPDH + NAD derivative – ''Leishmania mexicana''<BR />
[[1i32]], [[1i33]] – LmGAPDH + inhibitor<BR />
[[1i32]], [[1i33]] – LmGAPDH + inhibitor<BR />
[[1a7k]] – LmGAPDH + NAD<BR />
[[1a7k]] – LmGAPDH + NAD<BR />
-
[[1cer]], [[2g82]] - GAPDH + NAD – Thermus aquaticus<BR />
+
[[1cer]], [[2g82]] - GAPDH + NAD – ''Thermus aquaticus''<BR />
[[1gd1]] - GsGAPDH + NAD<BR />
[[1gd1]] - GsGAPDH + NAD<BR />
[[1npt]], [[1nq5]], [[1dbv]], [[2dbv]], [[3dbv]], [[4dbv]] - GsGAPDH (mutant) + NAD<BR />
[[1npt]], [[1nq5]], [[1dbv]], [[2dbv]], [[3dbv]], [[4dbv]] - GsGAPDH (mutant) + NAD<BR />
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''GAPDH ternary complex''
''GAPDH ternary complex''
-
[[3id5]], [[3dmt]], [[1ml3]] – TcGAPDH + NAD + inhibitor – Trypanosoma cruzi<BR />
+
[[3id5]], [[3dmt]], [[1ml3]] – TcGAPDH + NAD + inhibitor – ''Trypanosoma cruzi''<BR />
[[1qxs]] - TcGAPDH + NAD + G3P analog<BR />
[[1qxs]] - TcGAPDH + NAD + G3P analog<BR />
[[3cif]] - CpGAPDH + NAD + G3P<BR />
[[3cif]] - CpGAPDH + NAD + G3P<BR />
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===NADP-dependent specific aldehydes ALDH===
===NADP-dependent specific aldehydes ALDH===
-
[[3k2w]] - Betaine-ALDH – Pseudoalteromonas atlantica<BR />
+
[[3k2w]] - Betaine-ALDH – ''Pseudoalteromonas atlantica''<BR />
[[2hg2]], [[2opx]] – Lactaldehyde-EcALDH <BR />
[[2hg2]], [[2opx]] – Lactaldehyde-EcALDH <BR />
[[2o2p]] – Formyltetrahydrofolate-rALDH C-terminal
[[2o2p]] – Formyltetrahydrofolate-rALDH C-terminal
-
Binary complexes
+
''Binary complexes''
[[2wox]], [[2wme]] - Betaine-PaALDH + NADP<BR />
[[2wox]], [[2wme]] - Betaine-PaALDH + NADP<BR />
-
[[3lv1]] – Benzaldehyde-PpALDH3 + NADP – Pseudomonas putida<BR />
+
[[3lv1]] – Benzaldehyde-PpALDH3 + NADP – ''Pseudomonas putida''<BR />
[[2ilu]] - Lactaldehyde-EcALDH + NADP<BR />
[[2ilu]] - Lactaldehyde-EcALDH + NADP<BR />
[[2o2q]], [[2o2r]] - Formyltetrahydrofolate-rALDH C-terminal + NADP<BR />
[[2o2q]], [[2o2r]] - Formyltetrahydrofolate-rALDH C-terminal + NADP<BR />
-
[[3b4w]] - MtALDH + NADP – Mycobacterium tuberculosis<BR />
+
[[3b4w]] - MtALDH + NADP – ''Mycobacterium tuberculosis''<BR />
-
[[1eyy]], [[1ez0]] – ALDH + NADP – Vibrio harveyi
+
[[1eyy]], [[1ez0]] – ALDH + NADP – ''Vibrio harveyi''
-
Ternary complexes
+
''Ternary complexes''
[[3lns]] - Benzaldehyde-PpALDH3 + NADP + benzoate<BR />
[[3lns]] - Benzaldehyde-PpALDH3 + NADP + benzoate<BR />
[[2imp]] - Lactaldehyde-EcALDH + NADH + lactate<BR />
[[2imp]] - Lactaldehyde-EcALDH + NADH + lactate<BR />
-
[[2vro]] – ALDH + NADP + alcohol – Burkholderia xenovorans
+
[[2vro]] – ALDH + NADP + alcohol – ''Burkholderia xenovorans''
===NADP-dependent glyceraldehydes-3-phosphate dehydrogenase===
===NADP-dependent glyceraldehydes-3-phosphate dehydrogenase===
-
[[1euh]] - SmGAPDH – Streptococcus mutans<BR />
+
[[1euh]] - SmGAPDH – ''Streptococcus mutans''<BR />
[[1qi6]] - SmGAPDH (mutant)
[[1qi6]] - SmGAPDH (mutant)
-
GAPDH binary complex
+
''GAPDH binary complex''
[[2ehu]] - SmGAPDH + NADP<BR />
[[2ehu]] - SmGAPDH + NADP<BR />
-
[[2yyy]] – GAPDH + NADP – Methanocaldococcus jannaschii<BR />
+
[[2yyy]] – GAPDH + NADP – ''Methanocaldococcus jannaschii''<BR />
[[2pkq]] - spGAPDH subunit A, B + NADP – spinach<BR />
[[2pkq]] - spGAPDH subunit A, B + NADP – spinach<BR />
[[2pkr]], [[1rm4]], [[1jn0]] - spGAPDH subunit A + NADP<BR />
[[2pkr]], [[1rm4]], [[1jn0]] - spGAPDH subunit A + NADP<BR />
[[1rm3]], [[1rm5]] - spGAPDH subunit A (mutant) + NADP<BR />
[[1rm3]], [[1rm5]] - spGAPDH subunit A (mutant) + NADP<BR />
-
[[2czc]] – GAPDH + NADP – Pyrococcus horikoshii
+
[[2czc]] – GAPDH + NADP – ''Pyrococcus horikoshii''
-
GAPDH ternary complex
+
''GAPDH ternary complex''
[[2esd]], [[1qi1]] – SmGAPDH (mutant) + NADP + G3P<BR />
[[2esd]], [[1qi1]] – SmGAPDH (mutant) + NADP + G3P<BR />

Revision as of 11:07, 27 April 2011

Image:1nzx.png
Crystal Structure of Aldehyde dehydrogenase, 1nzx

Template:STRUCTURE 1nzx

Aldehyde dehydrogenase (ALDH) converts aldehydes to carboxylic acids while reducing NAD+ to NADH. In mammals there are 3 classes of ALDH and each contain constitutive and inducible forms. Class 1 is cytosolic; class 2 mitochondrial; class 3 found in tumors, stomach and cornea). ALDH family 7 member A1 is known as antiquitin and functions in the detoxification of aldehydes. Glyceraldehyde-3-phophate (G3P)-ALDH is called GAPDH.

3D Structures of Aldehyde dehydrogenase

ALDH class 1

3fx4 – ALDH1 + NADP + inhibitor – pig
1bxs – ALDH1 - sheep

ALDH class 2

3n80, 1o05 – hALDH2 residues 18-517- human
3n81, 2onn, 2ono, 1zum – hALDH2 residues 18-517 (mutant)
1ag8 – bALDH NAD binding domain - bovine

Binary complex

3inj, 3inl - hALDH2 residues 18-517 + agonist
2vle - hALDH2 residues 24-517 + daidzin
1nzx, 1nzz, 1o00, 1o02, 1cw3 - hALDH2 residues 18-517 + NAD
3n82, 3n83, 2onm, 2onp, 1nzw, 1o04 – hALDH2 residues 18-517 (mutant) + NADH
1a4z – bALDH2 NAD-binding domain + NAD

Ternary complex

1o01 - hALDH2 residues 18-517 + NADH + aldehyde

ALDH class 3

1ad3 – rALDH - rat

NAD-dependent specific aldehydes ALDH

2w8n, 2w8o, 2w8r – Succinate–semi-hALDH family 5
2w8p - Succinate–semi-hALDH family 5 (mutant)
3r31 – Betaine-ALDH – Agrobacterium tumefaciens
3ed6 - Betaine-SaALDH – Staphylococcus aureus
1a4s - Betaine-cALDH - cod
3llg – Aspartate-semi-MtALDH
2cq8 - Formyltetrahydrofolate-hALDH PP-binding domain - NMR
2nac – Formate-PsALDH
3i44 – ALDH – Bartonella henselae
3ek1 – ALDH – Brucella melitensis

Binary complexes

2w8q - Succinate–semi-hALDH family 5 + succinic acid
3fg0 - Betaine-SaALDH + NAD
1bpw - Betaine-cALDH + NAD
2j6l – Aminoadipate-semi-hALDH family 7 + NAD
2cfi - Formyltetrahydrofolate-hALDH hydrolase domain + formyltetrahydropterin
2nad - Formate-PsALDH + NAD – Pseudomonas
1bi9 – Retinal-rALDH2 + NAD
3l4p, 1sij – ALDH + AsO3 – Desulfovibrio gigas

Ternary complexes

2gsd – Formate-ALDH + NAD + azide – Moraxella
1nvm – Acetaldehyde-PsALDH + 4-hydroxy-oxovalerate aldolase + NAD
1llu - PaALDH + NAD + alcohol – Pseudomonas aeruginosa

NAD-dependent glyceraldehydes-3-phosphate dehydrogenase

1vsu – CpGAPDH – Cryptosporidium parvum
1ywg – PfGAPDH – Plasmodium falciparum
3cmc - GAPDH – Bacillus stearothermophilus
2ep7 – GAPDH – Aquifex aeolicus
2i5p – GAPDH – Kluyveromyces marxianus
1obf – GAPDH – Achromobacter xylosoxidans
1crw – PvGAPDH – Palinurus versicolor
1b7g – GAPDH – Sulfolobus solfataricus
2gd1 – GsGAPDH – Geobacillus stearothermophilus
3e6a – OsGAPDH – Oryza sativa
4gpd, 1gpd – GAPDH – lobster
3l6o, 3lc7 – SaGAPDH
1dc3, 1dc5, 1s7c – EcGAPDH - Escherichia coli

GAPDH binary complex

1k3t - TcGAPDH + inhibitor
3hja - GAPDH + NAD – Borellia burgdorferi
1vsv, 3cps - CpGAPDH + NAD
2vyn, 2vyv - rGAPDH residues 102-432 + EcGAPDH + NAD
2b4r, 2b4t – PfGAPDH (mutant) + NAD
1j0x – GAPDH + NAD – rabbit
1ihx – PvGAPDH + NAD
1dss, 1szj - PvGAPDH NAD-binding and catalytic domains+ NAD
1ihy – PvGAPDH + ADP-ribose
1gyq, 1gyp - LmGAPDH + NAD derivative – Leishmania mexicana
1i32, 1i33 – LmGAPDH + inhibitor
1a7k – LmGAPDH + NAD
1cer, 2g82 - GAPDH + NAD – Thermus aquaticus
1gd1 - GsGAPDH + NAD
1npt, 1nq5, 1dbv, 2dbv, 3dbv, 4dbv - GsGAPDH (mutant) + NAD
3e5r – OsGAPDH + NAD
3k73, 3lvf – SaGAPDH + NAD
3lc2 - SaGAPDH + G3P
3k9q, 3ksd, 3lc1, 3hq4 - SaGAPDH (mutant) + NAD
3gpd, 1u8f, 1znq – hGAPDH + NAD
1gad, 1dc6 - EcGAPDH + NAD
1gae - EcGAPDH (mutant) + NAD
1dc4 – EcGAPDH + G3P

GAPDH ternary complex

3id5, 3dmt, 1ml3 – TcGAPDH + NAD + inhibitor – Trypanosoma cruzi
1qxs - TcGAPDH + NAD + G3P analog
3cif - CpGAPDH + NAD + G3P
1nqa, 1nqo - GsGAPDH (mutant) + NAD + G3P
3ksz, 3kv3, 3l4s - SaGAPDH (mutant) + NAD + G3P

NADP-dependent specific aldehydes ALDH

3k2w - Betaine-ALDH – Pseudoalteromonas atlantica
2hg2, 2opx – Lactaldehyde-EcALDH
2o2p – Formyltetrahydrofolate-rALDH C-terminal

Binary complexes

2wox, 2wme - Betaine-PaALDH + NADP
3lv1 – Benzaldehyde-PpALDH3 + NADP – Pseudomonas putida
2ilu - Lactaldehyde-EcALDH + NADP
2o2q, 2o2r - Formyltetrahydrofolate-rALDH C-terminal + NADP
3b4w - MtALDH + NADP – Mycobacterium tuberculosis
1eyy, 1ez0 – ALDH + NADP – Vibrio harveyi

Ternary complexes

3lns - Benzaldehyde-PpALDH3 + NADP + benzoate
2imp - Lactaldehyde-EcALDH + NADH + lactate
2vro – ALDH + NADP + alcohol – Burkholderia xenovorans

NADP-dependent glyceraldehydes-3-phosphate dehydrogenase

1euh - SmGAPDH – Streptococcus mutans
1qi6 - SmGAPDH (mutant)

GAPDH binary complex

2ehu - SmGAPDH + NADP
2yyy – GAPDH + NADP – Methanocaldococcus jannaschii
2pkq - spGAPDH subunit A, B + NADP – spinach
2pkr, 1rm4, 1jn0 - spGAPDH subunit A + NADP
1rm3, 1rm5 - spGAPDH subunit A (mutant) + NADP
2czc – GAPDH + NADP – Pyrococcus horikoshii

GAPDH ternary complex

2esd, 1qi1 – SmGAPDH (mutant) + NADP + G3P

Antiquitin

2jg7 – Antiquitin – Acanthopagrus schlegeli

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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